BIOSENSOR – IC MONTELIBRETTI

Left to Right : Viviana Scognamiglio and Amina Antonacci

 

 

 DESCRIPTION: 

The research activity aims at the identification and characterization of biological systems of natural and synthetic origin for the development of biosensors to be applied in the agrifood, environmental and biomedical sectors:

– optical biosensors based on the green alga Chlamydomonas reinhardtii for chemical weapons monitoring

– electrochemical biosensors based on the green alga Chlamydomonas reinhardtii for the detection of nano-herbicides

– use of new biocomponent deposition techniques (enzymes, algae) on different substrates (e.g. ITO, screen-printed electrodes) including the electron spray ionization technique (ESI)

– use of state-of-the-art bionanotechnologies for the development of new artificial biocomponents (peptides bioinspired to the C. reinhardtii photosystem protein D1) and the use of functional materials (e.g. hydrogel, paper) and nanomaterials (e.g. graphene, carbon black) for the design of sustainable biosensors

 

Reference Persons :

VIVIANA SCOGNAMIGLIO
tel: 06 90672922
e-mail: viviana.scognamiglioATmlib.ic.cnr.it

AMINA ANTONACCI
tel: +39 06 90672617
e-mail: amina.antonacciATic.cnr.it

 
 
 
 FACILITIES AND LABORATORIES: 

The biosensor laboratory is equipped with electrochemical, optical, and spectrophotometric instruments: potentiostats, spectrofluorimeters and microfluidic devices.

In particular, the structure allows:

– studies of fluorescence and UV / VIS spectroscopy for biophysical characterization and the study of interaction between biological macromolecules, as well as the development of natural or biomimetic bioreceptors;

– labeling with fluorescent probes, immobilization on different supports (e.g. electrodes printed on paper), modification with nanomaterials (e.g. carbon black), synthesis of biological hybrids for the construction of optoelectronic devices;

– optimization of electro-optical transduction systems.

 

Lab Biosensor (reference: Viviana Scognamiglio)

The biosensor laboratory is equipped with electrochemical, optical, and spectrophotometric instruments: potentiostats, spectrofluorimeters and microfluidic devices.

In particular, the structure allows:

– studies of fluorescence and UV / VIS spectroscopy for biophysical characterization and the study of interaction between biological macromolecules, as well as the development of natural or biomimetic bioreceptors;

– labeling with fluorescent probes, immobilization on different supports (e.g. electrodes printed on paper), modification with nanomaterials (e.g. carbon black), synthesis of biological hybrids for the construction of optoelectronic devices;

– optimization of electro-optical transduction systems.

 

Lab Algae Biotechnology (responsible Amina Antonacci)

The Lab Algae Biotechnology is equipped with biological fume hoods, cell culture incubator, cell counters, optical microscopy, fresh- and sea- water algal strains, spectrophotometer, fluorimeter, and oxygraph.

In particular, the structure allows:

  • studies of characterization of algae strains related to growth curves, content of pigments, main photosynthetic parameters (e.g. FV/M and 1-Vj);
  • algal bioremediation tests on xenobiotic compounds;
  • analysis of global water toxicity exploiting algae cells vitality;
  • selection and optimization of biorecognition elements algae-based for biosensors development and environment monitoring.

 

 
 
 RELATED PROJECTS: 

2019-2020. INTERREG Ita-Hr Asse 3 OS 3.3. Titolo del Progetto: Managed use of treated urban wastewater for the Quality of the Adriatic Sea. Acronimo: AdSWiM. Coordinatore: Dr. Sabina Susmel, Università di Undine, Italia. Responsabile IC-CNR: Viviana Scognamiglio.

2018-2019. Accordo di cooperazione scientifica CNR/CNRST (Italia-Marocco), Programma di attività per il biennio 2018-2019. Titolo del Progetto: Alghe fotosintetiche e carbon black in reciproca congiunzione per lo sviluppo di sensori elettrochimici miniaturizzati per il monitoraggio sostenibile dell’erbicida atrazina in acque superficiali. Responsabile IC-CNR: Viviana Scognamiglio. Responsabile CNRST: Prof. Aziz Amine, Université Hassan II – Mohammedia, Morocco.

2017-2020. ERANETMED. Call for research proposals on Environmental challenges and solutions for vulnerable communities RQ3-2016 “Climate/demographic change” and Environment. Titolo Progetto: “Integrated nanotechnologies for sustainable sensing water and sanitation” (NanoSWS). Coordinatore: F. Arduini (Tor Vergata). Responsabile IC-CNR: Viviana Scognamiglio.

ERANETMED. Call for research proposals on Environmental challenges and solutions for vulnerable communities RQ3-2016 “Climate/demographic change” and Environment. Titolo Progetto: “Integrated nanotechnologies for sustainable sensing water and sanitation” (NanoSWS). Coordinatore: F. Arduini (Tor Vergata), Responsabile IC: Viviana Scognamiglio.

EUAlgae Action No. ES1408 (European Network for Algal-Bioproducts), European COST programme. Grant Holder: Fundación IMDEA Energía (Spain). Responsabile IC: Viviana Scognamiglio, parte del Working Group 3: Refining of Microalgae Into Its Value Components (http://eualgae.eu/working-groups/wg-3/) e del Working Group 4: Valorisation of Intermediates and Subproducts (http://eualgae.eu/working-groups/wg-4/). 

Prin 2016 – MIUR. Securing and ensuring sustainable use of agriculture waste, co- and by-products: an integrated analytical approach combining mass spectrometry with health effect-based biosensing. Unità di ricerca di Prof. Moscone Dinia Danila Palma (Università Tor Vergata) – Coordinatore: Prof. Roda Aldo (Università di Bologna).

MIUR-DAAD Joint Mobility Program (PPP Italien). Rapid detection of Salmonella using a smart multiplexed impedimetric paper-based sensor. Unità di ricerca di Dr. Fabiana Arduini (Università Tor Vergata) – Coordinatore: Prof. Olfa Kanoun (Technische Universität Chemnitz, Germania).

 
 
 PUBLICATIONS: 

  • Attaallah, R., Antonacci, A., Mazzaracchio, V., Moscone, D., Palleschi, G., Arduini, F., … & Scognamiglio, V. (2020). Carbon black nanoparticles to sense algae oxygen evolution for herbicides detection: Atrazine as a case study. Biosensors and Bioelectronics, 112203.
  • Antonacci, A., Scognamiglio, V., Mazzaracchio, V., Caratelli, V., Fiore, L., Moscone, D., & Arduini, F. (2020). Paper-based electrochemical devices for pharmaceutical field: State of the art and Perspectives. Frontiers in Bioengineering and Biotechnology, 8, 339.
  • Arduini, F., Cinti, S., Mazzaracchio, V., Scognamiglio, V., Amine, A., & Moscone, D. (2020). Carbon black as an outstanding and affordable nanomaterial for electrochemical (bio) sensor design. Biosensors and Bioelectronics, 112033.
  • Bartolucci, C., Antonacci, A., Arduini, F., Moscone, D., Fraceto, L., Campos, E., & Santander, J. M. P. (2020). Green nanomaterials fostering agrifood sustainability. TrAC Trends in Analytical Chemistry, 115840.
  • Arduini, F., Micheli, L., Scognamiglio, V., Mazzaracchio, V., & Moscone, D. (2020). Sustainable materials for the design of forefront printed (bio) sensors applied in agrifood sector. TrAC Trends in Analytical Chemistry, 115909.
  • Antonacci Amina and Viviana Scognamiglio. “Photosynthesis-based hybrid nanostructures: electrochemical sensors and photovoltaic cells as case studies.” TrAC Trends in Analytical Chemistry (2019).
  • Scognamiglio, V., Antonacci, A., Arduini, F., Moscone, D., Campos, E. V., Fraceto, L. F., Palleschi, G. (2019). An eco-designed paper-based algal biosensor for nanoformulated herbicide optical detection. Journal of Hazardous Materials.
  • Antonacci A., Arduini F., Moscone D., Palleschi G., Scognamiglio V. (2017) Nanostructured (bio)sensors for smart agriculture. TrAC, Trends in Analytical Chemistry98, 95-103.
  • Antonacci A., Lambreva M. D., Arduini F., Moscone D., Palleschi G., Scognamiglio V. (2017) A whole cell optical bioassay for the detection of chemical warfare mustard agent simulants. Sensors and actuators B: Chemical257, 658-665.
  • Scognamiglio, V., Rea, G., Arduini, F., & Palleschi, G. (2016).Biosensors for Sustainable Food-New Opportunities and Technical Challenges (Vol. 74).
  • Scognamiglio, V., Antonacci, A., Lambreva, M. D., Litescu, S. C., & Rea, G. (2015). Synthetic biology and biomimetic chemistry as converging technologies fostering a new generation of smart biosensors. Biosensors and Bioelectronics, 74, 1076-1086.
  • Scognamiglio, V., Arduini, F., Palleschi, G., & Rea, G. (2014). Biosensing technology for sustainable food safety. TrAC Trends in Analytical Chemistry, 62, 1-10.
  •  Antonacci*, A., & Scognamiglio*, V. (2019). Biotechnological Advances in the Design of Algae-Based Biosensors. Trends in biotechnology.
  •  Attaallah, R., Antonacci, A., Arduini, F., Amine, A., Scognamiglio* V. (2019) Nanobiosensors for bioclinical applications: pros and cons, in Green Nanoparticles – Synthesis and Biomedical Applications, Eds. Jayanta Kumar Patra, Gitishree Das, Leonardo F. Fraceto, Estefânia Vangelie Ramos Campos, published by Springer Nature Switzerland, pp. 117-149.
  •  Castrovilli, M. C., Bolognesi, P., Chiarinelli, J., Avaldi, L., Calandra, P., Antonacci, A., Scognamiglio*, V. (2019). The convergence of forefront technologies in the design of laccase-based biosensors-an update. TrAC Trends in Analytical Chemistry.