GAGS

GAGS: A Rapid High Performance Biosensor based on Gold Nanoparticle Anchored Graphene Sheets for the Electrochemical Detection of Biomarkers and DNA mutations for clinical and environmental applications.

24k€ 2016-2018 Bilateral Project with India (CNR-CSIR):

Graphene Gold Platform

In this work, we intentionally perform the electrochemical deposition of gold nanoparticles (AuNPs) onto graphene nanosheets (GN) in order to demonstrate a reliable and a stable approach to immobilize various biomolecules through a noble metal on any graphene based bio-device. Enzymes, antibodies and oligonucleotide immobilisation (DNA fragments, 10-30 bases) onto our graphene-gold nanoparticle platform will be tested with several electrochemical techniques includng Electrochemical Impedance Spectroscopy (EIS) for specific analytes of environmental interest (air pollution, indoor, outdoor) and/or food (mercury speciation, amphetamines, nicotine, PAH) in order to investigate the behavior of a wide range of biomolecules for future applications in the field of label free biosensors.

Malvano F, Albanese D, Pilloton R, Di Matteo M,Crescitelli A, (2017) A New Label-Free Impedimetric Affinity Sensor Based on Cholinesterases for Detection of Organophosphorous and Carbamic Pesticides in Food Samples: Impedimetric Versus Amperometric Detection; Food Bioprocess Technol; DOI 10.1007/s11947-017-1955-7
Malvano F, Albanese D, Pilloton R, Di Matteo M, (2017) A new label-free impedimetric aptasensor for gluten detection. Food Control Volume 79, September 2017, Pages 200-206 DOI 10.1016/j.foodcont.2017.03.033
Impedimetric Label-Free Immunosensor on Disposable Modified Screen-Printed Electrodes for Ochratoxin A by Francesca Malvano, Donatella Albanese, Alessio Crescitelli, Roberto Pilloton and Emanuela Esposito Biosensors 2016, 6(3), 33; doi:10.3390/bios6030033
A highly sensitive impedimetric label free immunosensor for Ochratoxin measurement in cocoa beans; (2016) Francesca Malvano, Donatella Albanese, Roberto Pilloton, Marisa Di Matteo; Food Chemistry Volume 212, 1 December 2016, Pages 688–694
Pilloton R (Editor) A special issue of Sensors (ISSN 1424-8220). Amperometric Biosensors (2016)

Buonasera K (Editor) Book of Abstract of the BBMEC12 International Workshop on Biosensors and BioAnalytical -techniques in Environmental, Food and Clinical Analysis held in Rome Sepetember 25th-29th, 2017, Chair R.Pilloton (ISBN 978 88 8080 245 7)

 ROBERTO PILLOTON, Area di Ricerca RM1, tel+393313291173, roberto.pillotonATcnr.it