Maria Teresa Giardi Personal Page
Short CV :
1976 : Degree with Laudem in Industrial Chemistry, Sapienza University of Rome, Italy
1976 : Centre of Mechanisms of Organic Reactions, CNR contract
1976-1977 : Teacher at state schools in Agronomy and Industry
1977-1982 : CNR research training fellowship on plant biochemistry at Institute of Physiology, CNR, Rome
1982 : Permanent position as a researcher, Institute of Biochemistry and Plant Physiology-CNR, Rome, Italy
1998 : Permanent position as primary researcher at Institute of Biochemistry and Plant Physiology-CNR, Rome, Italy
1998 on going : Permanent position as Manager of Research at Institute of Crystallography, CNR, Area of Research of Rome, Italy
Research Activity :
1992-1996: Responsible for Research Unit of Raisa-CNR target project on Plant Stress
1995-1997 : Coordinator of a Nato-Linkage Grant on “biosensors”
1997-1998: Coordinator of a Nato Computer Network between partner countries
1995-1996 : Responsible of EC project on “Signal transduction by protein phosphorylation”
1996-1998 : Co-responsible of EC project on “Mechanisms of photosynthetic electron transfer”
1997-2002: Responsible of a CNR target project on Biotechnology
1997: Responsible of a CNR technological transfer to industry
1999-2002: Responsible of a project for biosensors in monitoring extreme conditions, ASI
1999-2000: Responsible of a project for Italian Space Agency to study space stress on photosynthesis
2001-2004: Coordinator of EU project in life V frame on biosensors for environmental monitoring
2000-2008: Coordinator of European space agency (ESA) project for photosynthetic biodevices specific for Biopan/Foton ESA Space flight (2002, 2005, 2007).
2006-2009: Coordinator of EU project, STREP in collaboration with 6 EU countries, Nutra-Snacks for production of Nutraceuticas
2006-2009: Coordinator of Italian Space Agency project on Biotechnology for Anti-aging
2009: Partner in the Industrial project on Biosensors supported by Region (Bioal)
2009: Partner in three European Projects on industrial biosensors BEEP-C-EN, SENSBIOSYN and MULTIBIOPLAT
2007: Member of the network activities of European space scientists in the ESA project Ssioux
2009: Partner in the European Space Agency Project MARS 500 in collaboration with Russia
Selected publications :
Patents:
M.T. Giardi, D. Esposito, C. Leonardi, A. Mattoo, A. Margonelli, G. Angelini. Biosensor and method for the monitoring of pollutants (EU patent 2006, 01830148.1-2204)
MT Giardi, G Pezzotti. Multitrasduttore per la misura di fluorescenza ed amperometrica applicabile in sensoristica di sistemi biologici ed in biosensoristica: multiflamp. Registrato 30 Luglio 2006
G Rea, MT Giardi, G Pezzotti Multidispositivo portatile di tipo ottico per la misura della fluorescenza di marcatori di DNA. Registrato il 20 Dicembre 2006
G. Angelini, P. Ragni, D. Esposito, E. Pace, L. Pompili, M.T. Giardi (2000). Un biodosimetro per rivelare le radiazioni gamma (Brevetto, depositato 2000)
Editor, Invited Book Chapter :
M T Giardi. Photosynthetic proteins created by computational and biotechnological approaches in biosensing applications. In “Recognition receptors in biosensors”. Editor M Zourob, Publisher Springer Science (Invited Review for University Book of Montreal, in press)
MT Giardi, G Rea and Bruno Berra (Editors). Metabolites from Photosynthetic Organisms: Rationale for Production and Safety and Control technique. Landes Bioscience, Springer Publishers, Church St. Georgetown USA (in press)
M.T. Giardi and E. Piletska (Editors). Biotechnological Application of Photosynthetic Proteins: Biochips, Biosensors and Biodevices. (2006), Landes Bioscience, Springer Publishers, Church St. Georgetown USA. ISBN 1-58706-249-6. (Vendute circa 250 copie)
Publications :
M Damasso, T Dachev, G Falzetta, MT Giardi, G Rea and A Zanini The radiation environment observed by Liulin-Photo and R3D-B3 spectrum-dosimeters inside and outside Foton-M3 spacecraft. RADIATION MEASUREMENTS. ARTICLE IN PRESS http://www.sciencedirect.com/science
V Scognamiglio, D Raffi , M Lambreva , G Rea , A Tibuzzi , G Pezzotti , U Johanningmeier, MT Giardi . Chlamydomonas reinhardtii genetic variants as probes for fluorescence sensing system in detection of pollutants. ANAL BIOANAL CHEM. 2009 Feb 25. [Epub ahead of print] http://www.ncbi.nlm.nih.gov/pubmed/19238365
G Rea, D Esposito, M Damasso, A Serafini, A Margonelli, C Faraloni, G Torzillo, A Zanini, I Bertalan, U Johanningmeier and MT Giardi. Ionizing radiation impacts photochemical quantum yield and oxygen evolution activity of photosystem II in photosynthetic microorganisms. INTERNATIONAL JOURNAL RADIATION BIOLOGY Vol 84, Issue 11 2008 , pages 867 – 877
M. T. Giardi, M. Lambreva, G. Rea, V. Scognamiglio, A. Tibuzzi, T. Lavecchia, G. Pezzotti. A portable biosensor system for flow-assisted simultaneous detection of optical luminescence and amperometric current. LUMINESCENCE 2008; 23: 280-281
V. Scognamiglio, M. Lambreva, A. Antonacci, G. Rea, T. Lavecchia, I. Pezzotti and M. T. Giardi. A multi-array biosensor for fluorescence detection of pollulants. LUMINESCENCE 2008; 23: 267-268
M. Lambreva, G. Pappalettera, P. Boffi, A. Palasciano, V. Scognamiglio, G. Pezzotti, M. T. Giardi. Experimental laser-induced fluorescence sensor for remote detection of herbicide action on plants. LUMINESCENCE 2008; 23: 221-222
A. Tibuzzi, G. Rea, G. Pezzotti, D. Esposito, U. Johanningmeier and MT Giardi. (2007) A new miniaturized multiarrays biosensor system for fluorescence detection. JOURNAL OF PHYSICS: condensed matter 19, 395006
I Bertalan, D Esposito, G Torzillo, C Faraloni, U Johanningmeier, MT Giardi(2007). Photosystem II Stress Tolerance in the Unicellular Green Alga Chlamydomonas reinhardtii under Space Conditions, MICROGRAVITY SCI TECHNOL. XIX, 2
F. Breton, P. Euzet, S.A. Piletsky, M.T. Giardi, R. Rouillon. (2006) Integration of photosynthetic biosensor with molecularly imprinted polymer-based solid phase extraction cartridge ANALYTICA CHIMICA ACTA 2006, vol. 569, no1-2, 50-57
D. Esposito, C Faraloni, A Margonelli, E Pace, G Torzillo, A Zanini, MT Giardi. (2006) The effect of ionizing radiation on photosynthetic oxygenic microrganisms for survival in Space flight revealed by automatic Photosystem II-based biosensors. MICROGRAVITY SCI. TECHNOL. XVIII-3/4
M.T. Giardi, L Guzzella, P Euzet, R Rouillon, D Esposito (2005). Detection of herbicide subclasses by an optical multibiosensor based on an array of Photosystem II mutants. ENVIRON. SCI. TECHNOL. 39, 5378-5384
M.T. Giardi and E. Pace (2005). Photosynthetic proteins for technological applications. TRENDS IN BIOTECHNOLOGY, 25, 253-267
P. Euzet, M.T. Giardi, R Rouillon (2005). A crosslinked matrix of thylakoids coupled to the fluorescence transducer in order to detect herbicides. ANALYTICA CHIMICA ACTA, 539, 263-269
E. Touloupakis, L. Giannoudi, S.A. Piletsky, L. Guzzella, F. Pozzoni, M.T. Giardi (2005) A multi-biomediator based on immobilized Photosystem II on screen-printed electrodes for the detection. BIOSENSORS AND BIOELECTRONICS, 20 (10), 1984-1992
D. Esposito, E. Pace, A. Margonelli, M. Rizzuto, P. Giardi, M.T. Giardi (2002). A biodosemeter for the detection of ionising radiation utilising isolated enzymes. RADIATION PROTECTION DOSIMETRY. Vol 99, 303-305
M. Koblížek, J. Malý, J. Masojídek, J. Komenda, T. Kucera, M.T. Giardi, A.K. Mattoo, R. Pilloton (2002). A biosensor for the detection of triazine and phenylurea herbicides designed using Photosystem II coupled to a screen-printed electrode. BIOTECHNOLOGY AND BIOENGINEERING 78, 110-116
E. Pace, L. Pompili, A. Margonelli, P. Giardi and M.T. Giardi (2001). Pulse-chase experiments with 35S-methionine show D1 reaction II protein turnover in variously herbicide tolerant species. PESTICIDE BIOCHEMISTRY AND PHYSIOLOGY 69, 92-99
M.T. Giardi, M. Koblízek and J. Masojídek (2001). Photosystem II-based biosensors for detection of pollutants. BIOSENSORS AND BIOELECTRONICS 1027-1033
G. Angelini, P. Ragni, D. Esposito, P. Giardi, M.L. Pompili, R. Moscardelli, M.T. Giardi. A device to study the effect of space radiation on photosynthetic organisms. PHYSICA MEDICA. EUROPEAN JOURNAL OF MEDICAL PHYSICS. 2001, 267-268
A. Mattoo, M.T. Giardi, A. Raskind and M. Edelman (1999). Dynamic metabolism of photosystem II reaction center proteins and pigments. A review. PHYSIOL PLANT 107, 454-461
E. Franco, S. Alessandrelli, J. Masojidek, A. Margonelli and M.T. Giardi 1999. Modulation of D1 protein turnover under cadmium and heat stresses monitored by [35S] methionine incorporation. PLANT SCIENCE, 144, 53-61
B. Geiken, J. Masojídek, M. Rizzuto, M.L. Pompili and MT Giardi 1998. Incorporation of [35S]methionine in higher plants reveals that stimulation of the D1 reaction centre II protein turnover accompanies tolerance to heavy metal stress. PLANT, CELL AND ENVIRONMENT, 21, 1265-1273 M. Koblizek, J. Masojidek, J. Komenda, T. Kucera, R. Pilloton, A.K. Mattoo, and M.T. Giardi (1998). An ultrasensitive PSII-based biosensor for monitoring a class of photosynthetic herbicides. BIOTECHNOLOGY AND BIOENGINEERING, 60, 664-669
M.T. Giardi, J. Masojidek , and D. Godde. (1997). Effects of stresses on the turnover of D1 reaction centre II protein: review, PHYSIOL PLANT 101:635-642
M.T. Giardi, A. Cona, T. Kucera, B. Geiken, J. Masojidek, and A.K. Mattoo (1996). Long-term drought stress induces structural and functional reorganization of Photosystem II. PLANTA 199: 118-125
M.T. Giardi, T. Kucera, J.M. Briantais, and M. Hodges (1995). Decreased photosystem II core phosphorylation in a yellow-green mutant of wheat showing monophasic fluorescence induction curve. PLANT PHYSIOL, 109, 1059-10683
M.T. Giardi, A. Cona and B. Geiken (1995). Photosystem II core phosphorylation heterogeneity and the regulation of electron transfer in higher plants: review. BIOELECTROCHEM AND BIOENERGETICS 38, 67-75
M.T. Giardi, J. Komenda and J. Masojidek (1994). Role of protein phosphorylation on the sensitivity of photosystem II to strong illumination. PHYSIOL PLANT, 92, 181-187
M.T. Giardi (1993) Phosphorylation and disassembly of photosystem II as an early stage of photoinhibition. PLANTA, 190, 107-113
M.T. Giardi (1993) Significance of Photosystem II core phosphorylation heterogeneity for the herbicide binding domain. Z. NATURFORSCH, 48c, 241-245
M.T. Giardi, F. Rigoni and R. Barbato (1992). Q-B site modification and the binding of PSII-directed inhibitors during photoinhibition process. PHOTOSYNTHETI¬CA 27(1), 173-182
M.T. Giardi, F. Rigoni and R. Barbato (1992). Photosystem II core phosphory¬lation heterogeneity, differential herbicide binding, and regulation of electron transfer in photosystem II preparations from spinach. PLANT PHYSIOLOGY, 100, 1948-1054
M.T. Giardi, F. Rigoni, R. Barbato and G.M. Giacometti (1991). Relationships between heterogeneity of the photosystem II core complex from grana particles and phosphorylation. BIOCHEM BIOPHYS RES COMM 176 (3), 1298-1305
R. Barbato, F. Friso, M.T. Giardi, F. Rigoni, and G.M. Giacometti. (1991) Breakdown of the photosystem II reaction center D1 protein under photoinhibitory conditions: identification and localization of the C-terminal degradation products. BIOCHEMISTRY 30 (42), 10220-10226
M.C. Giardina, M.T. Giardi, M. De Agazio. (1990) Chlorsulfuron absorption and translocation in Zea mays. AGROCHIM, 4, 304-310
M.T. Giardi, J. Barber, M.C. Giardina, and R. Bassi. (1990) Studies on the herbicide binding site in isolated photosystem II core complexes from a flat-bed isoelectrofocusing method. Z. NATURFORSCH, 45, 366-372
G. Di Marco, N. D’Ambrosio, M.T. Giardi, A. Massacci and M Tricoli. (1989) Photosynthetic properties of leaves of a yellow green mutant of wheat compared to its wild type. PHOTOSYNT R ES, 21, 117-122
R. Barbato, F. Rigoni, M.T. Giardi, and G.M. Giacometti. (1989) The minor antenna complexes of an oxygen evolving photosystem II preparation: purification and stoichiometry. FEBS LETT, 251, 147-154
M.T. Giardi, J. Marder and J. Barber (1988) Herbicide binding to the isola¬ted photosystem II reaction centre. BIOCHIM ET BIOPHYS ACTA, 934, 64-71
D. Corradini, M.T. Giardi and A. Massacci (1987). Separation and quantita¬tion of ribulose-1,5-bisphosphate carboxylase-oxygenase in spinach leaves by high performance hydrophobic in-interaction chromatography. J. OF CHROMATOGRAPHY, 395, 523-529
M.T. Giardi, M.C. Giardina and G. Filacchioni (1985). Chemical-Biological degradation of primary metabolites of atrazine by a Nocardia strain. AGRIC AND BIOL CHEMISTRY, 49, 1551-1558
A. Massacci, M.T. Giardi, M. Tricoli and G. Di Marco (1986). Net photosynt¬hesis, C02 compensation point, dark respiration and relationships with ribulose-1,5-bisphosphate carboxylase activity in the flag leaf of two field grown wheats. CROP SCIENCE, 26, 557-563
M.C. Giardina, M. De Agazio, M.T. Giardi and R. Buffone (1983). Potassium uptake in atrazine treated roots of sensitive and resistant plants. PESTIC. BIO¬CHEM. AND PHYSIOL, 19, 11-14
A. Curulli, M.T. Giardi and G. Sleiter (1983). Electrophilic heteroaromatic substitutions. The reaction of some 3,4,5-tri- and 1,3,4,5-tetrasubstituted 2-methylpyrroles with Mannich reagents. GAZZETTA CHIMICA ITALIANA, 113, 115-122
M.C. Giardina, M.T. Giardi and G. Filacchioni (1982). Atrazine metabolism by Nocardia: elucidation of initial pathway and synthesis of potential metabolites. AGRIC AND BIOL CHEMISTRY. 46, 1439-1445
S. De Donatis, M.T. Giardi and G. Sleiter (1982). Electrophilic heteroaromatic substitutions. The acidi-catalized reaction of ethyl-3,4,5-trimethylpyrrile-2-carbo¬xylate with diazonium salts in aqueous acetic acid. J. ORGANIC CHEMISTRY, 47, 1750-1754
M.C. Giardina, M.T. Giardi and G. Filacchioni (1980). 4-amino -2-chloro-1,3,5-triazine: a new metabolite of atrazine by a soil bacteriun. AGRIC AND BIOLOGICAL CHEMISTRY, 44, 2067-2072
M.T. Giardi and G. Sleiter (1980). Electrophilic heteroaromatic substitutions. The reaction of formaldehyde with ethyl-3,4,5-trimethylphyrrole-2-carboxylate in acid solution. GAZZETTA CHIMICA ITALIANA, 110, 115-122
M.C. Giardina, M.T. Giardi and R. Buffone (1979). Soil enrichment studies with atrazine. Long term atrazine effects on degradation and microbiological composition. CHEMOSPHERE, 11/12, 831-834