Look at a list of IC publications: 2020, 2019, 2018, 2017, 2016, 2015, 2014
2020 |
|
Antonacci, Amina; Scognamiglio, Viviana Biotechnological Advances in the Design of Algae-Based Biosensors Journal Article Trends in biotechnology, 38 (3), pp. 334–347, 2020. @article{antonacci2020biotechnological, title = {Biotechnological Advances in the Design of Algae-Based Biosensors}, author = {Amina Antonacci and Viviana Scognamiglio}, doi = {10.1016/j.tibtech.2019.10.005}, year = {2020}, date = {2020-03-01}, journal = {Trends in biotechnology}, volume = {38}, number = {3}, pages = {334--347}, publisher = {Elsevier}, keywords = {}, pubstate = {published}, tppubtype = {article} } | |
Luca, Stefania De; Verdoliva, Valentina; Saviano, Michele; Fattorusso, Roberto; Diana, Donatella Journal of Peptide Science, 26 (2), pp. e3231, 2020, (e3231 PSC-19-0097.R2). @article{doi:10.1002/psc.3231, title = {SPR and NMR characterization of the molecular interaction between A9 peptide and a model system of HER2 receptor: A fragment approach for selecting peptide structures specific for their target}, author = {Stefania De Luca and Valentina Verdoliva and Michele Saviano and Roberto Fattorusso and Donatella Diana}, url = {https://onlinelibrary.wiley.com/doi/abs/10.1002/psc.3231}, doi = {10.1002/psc.3231}, year = {2020}, date = {2020-01-01}, journal = {Journal of Peptide Science}, volume = {26}, number = {2}, pages = {e3231}, abstract = {The binding process of A9 peptide toward HER2-DIVMP, a synthetic model of the receptor domain IV, was studied by using the surface plasmon resonance (SPR) technique, with the aim of validating it as a fast and reliable screening method for selecting peptide ligands specifically targeting a domain of their target. To investigate the structural basis of A9 binding to the model of HER2-DIVMP, multiple ligand-based nuclear magnetic resonance (NMR) methods were applied. The use of saturation transfer difference (STD) and WaterLOGSY NMR experiments identified key residues in the peptide for the receptor binding. Moreover, the bound conformation of the A9 peptide was obtained using transferred nuclear Overhauser effect spectroscopy (trNOESY) experiments. The NMR data revealed an extended binding surface that confirms an in silico model previously reported. These structural findings could provide good starting points for future lead structures optimization specific for the receptor target.}, note = {e3231 PSC-19-0097.R2}, keywords = {}, pubstate = {published}, tppubtype = {article} } The binding process of A9 peptide toward HER2-DIVMP, a synthetic model of the receptor domain IV, was studied by using the surface plasmon resonance (SPR) technique, with the aim of validating it as a fast and reliable screening method for selecting peptide ligands specifically targeting a domain of their target. To investigate the structural basis of A9 binding to the model of HER2-DIVMP, multiple ligand-based nuclear magnetic resonance (NMR) methods were applied. The use of saturation transfer difference (STD) and WaterLOGSY NMR experiments identified key residues in the peptide for the receptor binding. Moreover, the bound conformation of the A9 peptide was obtained using transferred nuclear Overhauser effect spectroscopy (trNOESY) experiments. The NMR data revealed an extended binding surface that confirms an in silico model previously reported. These structural findings could provide good starting points for future lead structures optimization specific for the receptor target. | |
Capelli, Davide; Parravicini, Chiara; Pochetti, Giorgio; Montanari, Roberta; Temporini, Caterina; Rabuffetti, Marco; Trincavelli, Maria Letizia; Daniele, Simona; Fumagalli, Marta; Saporiti, Simona; Bonfanti, Elisabetta; Abbracchio, Maria P; Eberini, Ivano; Ceruti, Stefania; Calleri, Enrica; Capaldi, Stefano Frontiers in Chemistry, 7 , pp. 910, 2020, ISSN: 2296-2646. @article{10.3389/fchem.2019.00910, title = {Surface Plasmon Resonance as a Tool for Ligand Binding Investigation of Engineered GPR17 Receptor, a G Protein Coupled Receptor Involved in Myelination}, author = {Davide Capelli and Chiara Parravicini and Giorgio Pochetti and Roberta Montanari and Caterina Temporini and Marco Rabuffetti and Maria Letizia Trincavelli and Simona Daniele and Marta Fumagalli and Simona Saporiti and Elisabetta Bonfanti and Maria P Abbracchio and Ivano Eberini and Stefania Ceruti and Enrica Calleri and Stefano Capaldi}, url = {https://www.frontiersin.org/article/10.3389/fchem.2019.00910}, doi = {10.3389/fchem.2019.00910}, issn = {2296-2646}, year = {2020}, date = {2020-01-01}, journal = {Frontiers in Chemistry}, volume = {7}, pages = {910}, abstract = {The aim of this study was to investigate the potential of surface plasmon resonance (SPR) spectroscopy for the measurement of real-time ligand-binding affinities and kinetic parameters for GPR17, a G protein-coupled receptor (GPCR) of major interest in medicinal chemistry as potential target in demyelinating diseases. The receptor was directly captured, in a single-step, from solubilized membrane extracts on the sensor chip through a covalently bound anti-6x-His-antibody and retained its ligand binding activity for over 24 h. Furthermore, our experimental setup made possible, after a mild regeneration step, to remove the bound receptor without damaging the antibody, and thus to reuse many times the same chip. Two engineered variants of GPR17, designed for crystallographic studies, were expressed in insect cells, extracted from crude membranes and analyzed for their binding with two high affinity ligands: the antagonist Cangrelor and the agonist Asinex 1. The calculated kinetic parameters and binding constants of ligands were in good agreement with those reported from activity assays and highlighted a possible functional role of the N-terminal residues of the receptor in ligand recognition and binding. Validation of SPR results was obtained by docking and molecular dynamics of GPR17-ligands interactions and by functional in vitro studies. The latter allowed us to confirm that Asinex 1 behaves as GPR17 receptor agonist, inhibits forskolin-stimulated adenylyl cyclase pathway and promotes oligodendrocyte precursor cell maturation and myelinating ability.}, keywords = {}, pubstate = {published}, tppubtype = {article} } The aim of this study was to investigate the potential of surface plasmon resonance (SPR) spectroscopy for the measurement of real-time ligand-binding affinities and kinetic parameters for GPR17, a G protein-coupled receptor (GPCR) of major interest in medicinal chemistry as potential target in demyelinating diseases. The receptor was directly captured, in a single-step, from solubilized membrane extracts on the sensor chip through a covalently bound anti-6x-His-antibody and retained its ligand binding activity for over 24 h. Furthermore, our experimental setup made possible, after a mild regeneration step, to remove the bound receptor without damaging the antibody, and thus to reuse many times the same chip. Two engineered variants of GPR17, designed for crystallographic studies, were expressed in insect cells, extracted from crude membranes and analyzed for their binding with two high affinity ligands: the antagonist Cangrelor and the agonist Asinex 1. The calculated kinetic parameters and binding constants of ligands were in good agreement with those reported from activity assays and highlighted a possible functional role of the N-terminal residues of the receptor in ligand recognition and binding. Validation of SPR results was obtained by docking and molecular dynamics of GPR17-ligands interactions and by functional in vitro studies. The latter allowed us to confirm that Asinex 1 behaves as GPR17 receptor agonist, inhibits forskolin-stimulated adenylyl cyclase pathway and promotes oligodendrocyte precursor cell maturation and myelinating ability. | |
2019 |
|
Muzzini, Valerio Giorgio; Mattioni, Michele; Calandrelli, Roberto; Leonardi, Luca; Simonetti, Marco; Righini, Guido; Pifferi, Augusto; Ianniello, Luca; Claudio, Angelo De Simone Maurizio Di; Giardini, Giorgio; Benedetti, Bruno; Filippone, Francesco Realizzazione di un sistema di videoconferenza utilizzando il software open source Jitsi. Journal Article Smart eLab, 13 , pp. 2-8, 2019, ISSN: 2282-2259. @article{Filippone2019, title = {Realizzazione di un sistema di videoconferenza utilizzando il software open source Jitsi. }, author = {Valerio Giorgio Muzzini and Michele Mattioni and Roberto Calandrelli and Luca Leonardi and Marco Simonetti and Guido Righini and Augusto Pifferi and Luca Ianniello and Angelo De Simone Maurizio Di Claudio and Giorgio Giardini and Bruno Benedetti and Francesco Filippone }, doi = {10.30441/smart-elab.v13i0.222}, issn = {2282-2259}, year = {2019}, date = {2019-12-03}, journal = {Smart eLab}, volume = {13}, pages = {2-8}, abstract = {Il sistema di videoconferenza \`{e} uno strumento utile per mantenere contatti e rapporti di lavoro tra soggetti distanti geograficamente tra loro. Nell’ambito del riordino e accorpamento intrapreso dall’amministrazione centrale del CNR \`{e} emersa la necessit\`{a} di poter aver uno strumento flessibile di videoconferenza. Il sistema Jitsi ha le caratteristiche di flessibilit\`{a}, semplicit\`{a} di installazione e uso per poter essere il sistema di riferimento per questo tipo di applicazione, oltre ad avere un codice sorgente aperto. Il documento descrive i test che sono stati fatti per poter installare e configurare un sistema di videoconferenza funzionante e gestito su server propri d’Istituto.}, keywords = {}, pubstate = {published}, tppubtype = {article} } Il sistema di videoconferenza è uno strumento utile per mantenere contatti e rapporti di lavoro tra soggetti distanti geograficamente tra loro. Nell’ambito del riordino e accorpamento intrapreso dall’amministrazione centrale del CNR è emersa la necessità di poter aver uno strumento flessibile di videoconferenza. Il sistema Jitsi ha le caratteristiche di flessibilità, semplicità di installazione e uso per poter essere il sistema di riferimento per questo tipo di applicazione, oltre ad avere un codice sorgente aperto. Il documento descrive i test che sono stati fatti per poter installare e configurare un sistema di videoconferenza funzionante e gestito su server propri d’Istituto. |  |
Righini, Guido; Zanotti, Gloria; Scibetta, Emanuele Vincenzo; Pifferi, Augusto Smart eLab, 13 , pp. 9-13, 2019, ISSN: 2282-2259. @article{Pifferi2019, title = {Progettazione e realizzazione della Mostra Divulgativa: "La Scienza dei Colori - I Colori della Scienza". Un esempio di percorso formativo di Alternanza Scuola Lavoro}, author = {Guido Righini and Gloria Zanotti and Emanuele Vincenzo Scibetta and Augusto Pifferi}, doi = {10.30441/smart-elab.v13i0.223}, issn = {2282-2259}, year = {2019}, date = {2019-12-03}, journal = {Smart eLab}, volume = {13}, pages = {9-13}, abstract = {La realizzazione di un evento scientifico \`{e} l'occasione per diffondere nella societ\`{a} le nuove conoscenze e le nuove metodologie prodotte dalla comunit\`{a} scientifica. Nel caso di eventi dedicati agli studenti, la metodologia "Divulgazione tra Pari" pu\`{o} essere scelta per migliorare l'efficacia della comunicazione. In questo articolo si descrive un'iniziativa di Alternanza Scuola Lavoro svolta presso un liceo scientifico con la finalit\`{a} di realizzare sia una mostra divulgativa, applicando questa modalit\`{a} comunicativa. Durante le fasi di realizzazione si \`{e} valutato l'efficacia del software di scrittura collaborativa online come strumento educativo. I risultati dell'esperienza sono riportati nell'articolo.}, keywords = {}, pubstate = {published}, tppubtype = {article} } La realizzazione di un evento scientifico è l'occasione per diffondere nella società le nuove conoscenze e le nuove metodologie prodotte dalla comunità scientifica. Nel caso di eventi dedicati agli studenti, la metodologia "Divulgazione tra Pari" può essere scelta per migliorare l'efficacia della comunicazione. In questo articolo si descrive un'iniziativa di Alternanza Scuola Lavoro svolta presso un liceo scientifico con la finalità di realizzare sia una mostra divulgativa, applicando questa modalità comunicativa. Durante le fasi di realizzazione si è valutato l'efficacia del software di scrittura collaborativa online come strumento educativo. I risultati dell'esperienza sono riportati nell'articolo. |  |
Righini, Guido Proposta di Modelli Tipografici LaTeX ottimizzati per prodotti editoriali del CNR Journal Article Smart eLab, 13 , pp. 14-17, 2019, ISSN: 2282-2259. @article{Righini2019, title = {Proposta di Modelli Tipografici LaTeX ottimizzati per prodotti editoriali del CNR }, author = {Guido Righini}, doi = {10.30441/smart-elab.v13i0.225}, issn = {2282-2259}, year = {2019}, date = {2019-12-03}, journal = {Smart eLab}, volume = {13}, pages = {14-17}, abstract = {In questo articolo vengono proposti dei modelli tipografici LaTeX per la realizzazione di prodotti editoriali accademici. Questi modelli sono stati ottimizzati per le specifiche esigenze degli istituti di ricerca del Consiglio Nazionale delle Ricerche. Sono stati progettati con la finalit\`{a} di rendere semplice il loro utilizzo ad autori neofiti del linguaggio di programmazione LaTeX.}, keywords = {}, pubstate = {published}, tppubtype = {article} } In questo articolo vengono proposti dei modelli tipografici LaTeX per la realizzazione di prodotti editoriali accademici. Questi modelli sono stati ottimizzati per le specifiche esigenze degli istituti di ricerca del Consiglio Nazionale delle Ricerche. Sono stati progettati con la finalità di rendere semplice il loro utilizzo ad autori neofiti del linguaggio di programmazione LaTeX. |  |
Ranieri, Antonello Studio del sistema Sole-Terra e realizzazione di un robot per l'inseguimento della traiettoria solare. Journal Article Smart eLab, 13 , pp. 18-23, 2019, ISSN: 2282-2259. @article{Ranieri2019, title = {Studio del sistema Sole-Terra e realizzazione di un robot per l'inseguimento della traiettoria solare. }, author = {Antonello Ranieri}, doi = {10.30441/smart-elab.v13i0.226}, issn = {2282-2259}, year = {2019}, date = {2019-12-03}, journal = {Smart eLab}, volume = {13}, pages = {18-23}, keywords = {}, pubstate = {published}, tppubtype = {article} } |  |
Castelli, Andrea; Biffi, Giulia; Ceseracciu, Luca; Spirito, Davide; Prato, Mirko; Altamura, Davide; Giannini, Cinzia; Artyukhin, Sergey; Krahne, Roman; Manna, Liberato; Arciniegas, Milena P Revealing Photoluminescence Modulation from Layered Halide Perovskite Microcrystals upon Cyclic Compression Journal Article Advanced Materials, 31 (1), pp. 1805608, 2019. @article{doi:10.1002/adma.201805608, title = {Revealing Photoluminescence Modulation from Layered Halide Perovskite Microcrystals upon Cyclic Compression}, author = {Andrea Castelli and Giulia Biffi and Luca Ceseracciu and Davide Spirito and Mirko Prato and Davide Altamura and Cinzia Giannini and Sergey Artyukhin and Roman Krahne and Liberato Manna and Milena P Arciniegas}, url = {https://onlinelibrary.wiley.com/doi/abs/10.1002/adma.201805608}, doi = {10.1002/adma.201805608}, year = {2019}, date = {2019-11-14}, journal = {Advanced Materials}, volume = {31}, number = {1}, pages = {1805608}, abstract = {Abstract Halide perovskites show promise for high-efficiency solar energy conversion and light-emitting diode devices owing to their bandgap, which falls within the visible optical range. However, due to their rigidity, GPa pressures are necessary to control the complex interplay between their electronic and crystallographic structure. Layered perovskites are likely to be controlled using much lower pressures by exploiting the optical anisotropy of the embedded organic molecules in the structure. This work introduces layered perovskite microplatelets and demonstrates the extreme sensitivity of their emission to cyclic mechanical loading in the range of tens of MPa. A drastic change in their emission is observed in situ, from near-white to an enhanced blue color. This process is reversible, as is evident from a hysteresis loop in the photoluminescence (PL) intensity of the microplatelets. A combination of experimental analysis and computational modelling shows that such behavior cannot be attributed to changes in the crystallographic structure of the flakes. Instead, it suggests that, thanks to their structural anisotropy, microplate alignment and reorientation are responsible for the observed PL modulation. The possibility to tune the optical emission of layered perovskite crystals via low pressures makes them highly interesting as active materials in applications where stress sensing or light modulation is desired.}, keywords = {}, pubstate = {published}, tppubtype = {article} } Abstract Halide perovskites show promise for high-efficiency solar energy conversion and light-emitting diode devices owing to their bandgap, which falls within the visible optical range. However, due to their rigidity, GPa pressures are necessary to control the complex interplay between their electronic and crystallographic structure. Layered perovskites are likely to be controlled using much lower pressures by exploiting the optical anisotropy of the embedded organic molecules in the structure. This work introduces layered perovskite microplatelets and demonstrates the extreme sensitivity of their emission to cyclic mechanical loading in the range of tens of MPa. A drastic change in their emission is observed in situ, from near-white to an enhanced blue color. This process is reversible, as is evident from a hysteresis loop in the photoluminescence (PL) intensity of the microplatelets. A combination of experimental analysis and computational modelling shows that such behavior cannot be attributed to changes in the crystallographic structure of the flakes. Instead, it suggests that, thanks to their structural anisotropy, microplate alignment and reorientation are responsible for the observed PL modulation. The possibility to tune the optical emission of layered perovskite crystals via low pressures makes them highly interesting as active materials in applications where stress sensing or light modulation is desired. | |
Nardella, Maria I; Rosato, Antonio; Belviso, Benny D; Caliandro, Rocco; Natile, Giovanni; Arnesano, Fabio Oxidation of Human Copper Chaperone Atox1 and Disulfide Bond Cleavage by Cisplatin and Glutathione Journal Article International journal of molecular sciences, 20 (18), pp. 4390, 2019. @article{nardella2019oxidation, title = {Oxidation of Human Copper Chaperone Atox1 and Disulfide Bond Cleavage by Cisplatin and Glutathione}, author = {Maria I Nardella and Antonio Rosato and Benny D Belviso and Rocco Caliandro and Giovanni Natile and Fabio Arnesano}, doi = {10.3390/ijms20184390 publisher=Multidisciplinary Digital Publishing Institute}, year = {2019}, date = {2019-09-09}, journal = {International journal of molecular sciences}, volume = {20}, number = {18}, pages = {4390}, keywords = {}, pubstate = {published}, tppubtype = {article} } | |
Terzi, Alberta; Gallo, Nunzia; Bettini, Simona; Sibillano, Teresa; Altamura, Davide; Campa, Lorena; Natali, Maria Lucia; Salvatore, Luca; Madaghiele, Marta; Caro, Liberato De; Valli, Ludovico; Sannino, Alessandro; Giannini, Cinzia Investigations of Processing–Induced Structural Changes in Horse Type-I Collagen at Sub and Supramolecular Levels Journal Article Frontiers in Bioengineering and Biotechnology, 7 , pp. 203, 2019, ISSN: 2296-4185. @article{10.3389/fbioe.2019.00203, title = {Investigations of Processing\textendashInduced Structural Changes in Horse Type-I Collagen at Sub and Supramolecular Levels}, author = {Alberta Terzi and Nunzia Gallo and Simona Bettini and Teresa Sibillano and Davide Altamura and Lorena Campa and Maria Lucia Natali and Luca Salvatore and Marta Madaghiele and Liberato De Caro and Ludovico Valli and Alessandro Sannino and Cinzia Giannini}, url = {https://www.frontiersin.org/article/10.3389/fbioe.2019.00203}, doi = {10.3389/fbioe.2019.00203}, issn = {2296-4185}, year = {2019}, date = {2019-08-26}, journal = {Frontiers in Bioengineering and Biotechnology}, volume = {7}, pages = {203}, abstract = {The aim of this work is to evaluate the effects of different extraction and material processing protocols on the collagen structure and hierarchical organization of equine tendons. Wide and Small Angle X-ray Scattering investigations on raw powders and thin films revealed that not only the extraction and purification treatments, but also the processing conditions may affect the extent of the protein crystalline domain and induce a nanoscale “shield effect.” This is due to the supramolecular fiber organization, which protects the atomic scale structure from the modifications that occur during fabrication protocols. Moreover, X-ray analyses and Fourier Transform Infrared spectroscopy performed on the biomaterial sheds light on the relationship between processing conditions, triple helical content and the organization in atomic and nanoscale domains. It was found that the mechanical homogenization of the slurry in acidic solution is a treatment that ensures a high content of super-organization of collagen into triple helices and a lower crystalline domain in the material. Finally, mechanical tensile tests were carried out, proving that the acidic solution is the condition which most enhances both mechanical stiffness and supramolecular fiber organization of the films.}, keywords = {}, pubstate = {published}, tppubtype = {article} } The aim of this work is to evaluate the effects of different extraction and material processing protocols on the collagen structure and hierarchical organization of equine tendons. Wide and Small Angle X-ray Scattering investigations on raw powders and thin films revealed that not only the extraction and purification treatments, but also the processing conditions may affect the extent of the protein crystalline domain and induce a nanoscale “shield effect.” This is due to the supramolecular fiber organization, which protects the atomic scale structure from the modifications that occur during fabrication protocols. Moreover, X-ray analyses and Fourier Transform Infrared spectroscopy performed on the biomaterial sheds light on the relationship between processing conditions, triple helical content and the organization in atomic and nanoscale domains. It was found that the mechanical homogenization of the slurry in acidic solution is a treatment that ensures a high content of super-organization of collagen into triple helices and a lower crystalline domain in the material. Finally, mechanical tensile tests were carried out, proving that the acidic solution is the condition which most enhances both mechanical stiffness and supramolecular fiber organization of the films. | |
Lucilla Pronti Martina Romani, Gianluca Verona-Rinati Ombretta Tarquini Francesco Colao Marcello Colapietro Augusto Pifferi Mariangela Cestelli-Guidi Marco Marinelli Hermitage, 2 (3), pp. 2275-2286, 2019. @article{Pronti2019, title = {Post-Processing of VIS, NIR, and SWIR Multispectral Images of Paintings. New Discovery on the The Drunkenness of Noah, Painted by Andrea Sacchi, Stored at Palazzo Chigi (Ariccia, Rome) }, author = { Lucilla Pronti, Martina Romani, Gianluca Verona-Rinati, Ombretta Tarquini, Francesco Colao, Marcello Colapietro, Augusto Pifferi, Mariangela Cestelli-Guidi, Marco Marinelli}, doi = {10.3390/heritage2030139}, year = {2019}, date = {2019-08-02}, journal = {Hermitage}, volume = {2}, number = {3}, pages = {2275-2286}, abstract = {IR Reflectography applied to the identification of hidden details of paintings is extremely useful for authentication purposes and for revealing technical hidden features. Recently, multispectral imaging has replaced traditional imaging techniques thanks to the possibility to select specific spectral ranges bringing out interesting details of the paintings. VIS\textendashNIR\textendashSWIR images of one of the The Drunkenness of Noah versions painted by Andrea Sacchi, acquired with a modified reflex and InGaAs cameras, are presented in this research. Starting from multispectral images we performed post-processing analysis, using visible and infrared false-color images and principal component analysis (PCA) in order to highlight pentimenti and underdrawings. Radiography was performed in some areas to better investigate the inner pictorial layers. This study represents the first published scientific investigation of The Drunkenness of Noah’s artistic production, painted by Andrea Sacchi.}, keywords = {}, pubstate = {published}, tppubtype = {article} } IR Reflectography applied to the identification of hidden details of paintings is extremely useful for authentication purposes and for revealing technical hidden features. Recently, multispectral imaging has replaced traditional imaging techniques thanks to the possibility to select specific spectral ranges bringing out interesting details of the paintings. VIS–NIR–SWIR images of one of the The Drunkenness of Noah versions painted by Andrea Sacchi, acquired with a modified reflex and InGaAs cameras, are presented in this research. Starting from multispectral images we performed post-processing analysis, using visible and infrared false-color images and principal component analysis (PCA) in order to highlight pentimenti and underdrawings. Radiography was performed in some areas to better investigate the inner pictorial layers. This study represents the first published scientific investigation of The Drunkenness of Noah’s artistic production, painted by Andrea Sacchi. | |
Giannini, Cinzia; Terzi, Alberta; Fusaro, Luca; Sibillano, Teresa; Diaz, Ana; Ramella, Martina; Lutz-Bueno, Viviane; Boccafoschi, Francesca; Bunk, Oliver Scanning X-ray microdiffraction of decellularized pericardium tissue at increasing glucose concentration Journal Article Journal of Biophotonics, 12 (10), pp. e201900106, 2019. @article{doi:10.1002/jbio.201900106, title = {Scanning X-ray microdiffraction of decellularized pericardium tissue at increasing glucose concentration}, author = {Cinzia Giannini and Alberta Terzi and Luca Fusaro and Teresa Sibillano and Ana Diaz and Martina Ramella and Viviane Lutz-Bueno and Francesca Boccafoschi and Oliver Bunk}, url = {https://onlinelibrary.wiley.com/doi/abs/10.1002/jbio.201900106}, doi = {10.1002/jbio.201900106}, year = {2019}, date = {2019-07-18}, journal = {Journal of Biophotonics}, volume = {12}, number = {10}, pages = {e201900106}, abstract = {Abstract Blood glucose supplies energy to cells and is critical for the human brain. Glycation of collagen, the nonenzymatic formation of glucose-bridges, relates to diseases of aging populations and diabetics. This chemical reaction, together with its biomechanical effects, has been well studied employing animal models. However, the direct impact of glycation on collagen nano-structure is largely overlooked, and there is a lack of ex vivo model systems. Here, we present the impact of glucose on collagen nanostructure in a model system based on abundantly available connective tissue of farm animals. By combining ex vivo small and wide-angle X-ray scattering (SAXS/WAXS) imaging, we characterize intra- and inter-molecular parameters of collagen in decellularized bovine pericardium with picometer precision. We observe three distinct regimes according to glucose concentration. Such a study opens new avenues for inspecting the effects of diabetes mellitus on connective tissues and the influence of therapies on the resulting secondary disorders.}, keywords = {}, pubstate = {published}, tppubtype = {article} } Abstract Blood glucose supplies energy to cells and is critical for the human brain. Glycation of collagen, the nonenzymatic formation of glucose-bridges, relates to diseases of aging populations and diabetics. This chemical reaction, together with its biomechanical effects, has been well studied employing animal models. However, the direct impact of glycation on collagen nano-structure is largely overlooked, and there is a lack of ex vivo model systems. Here, we present the impact of glucose on collagen nanostructure in a model system based on abundantly available connective tissue of farm animals. By combining ex vivo small and wide-angle X-ray scattering (SAXS/WAXS) imaging, we characterize intra- and inter-molecular parameters of collagen in decellularized bovine pericardium with picometer precision. We observe three distinct regimes according to glucose concentration. Such a study opens new avenues for inspecting the effects of diabetes mellitus on connective tissues and the influence of therapies on the resulting secondary disorders. | |
Caliandro, Rocco; Toson, Valentina; Palin, Luca; Conterosito, Eleonora; Aceto, Maurizio; Gianotti, Valentina; Boccaleri, Enrico; Dooryhee, Eric; Milanesio, Marco New Hints on the Maya Blue Formation Process by PCA-Assisted In Situ XRPD/PDF and Optical Spectroscopy Journal Article Chemistry – A European Journal, 25 (49), pp. 11503-11511, 2019. @article{doi:10.1002/chem.201901932, title = {New Hints on the Maya Blue Formation Process by PCA-Assisted In Situ XRPD/PDF and Optical Spectroscopy}, author = {Rocco Caliandro and Valentina Toson and Luca Palin and Eleonora Conterosito and Maurizio Aceto and Valentina Gianotti and Enrico Boccaleri and Eric Dooryhee and Marco Milanesio}, url = {https://chemistry-europe.onlinelibrary.wiley.com/doi/abs/10.1002/chem.201901932}, doi = {10.1002/chem.201901932}, year = {2019}, date = {2019-06-25}, journal = {Chemistry \textendash A European Journal}, volume = {25}, number = {49}, pages = {11503-11511}, abstract = {Abstract The exact recipe to prepare the ancient Maya Blue (MB), an incredibly resistant and brilliant pigment prepared from indigo (dye) and Palygorskite (clay), is lost to the ages. To unravel the key features of the MB formation process, several inorganic-dye couples were heated to 200 °C and cooled to RT, to investigate their reactivity and the diffusion and degree of sequestration of the dye into the inorganic host. In situ XRPD/PDF and fiber optic reflectance spectroscopy (FORS) data, along with TGA, provided a comprehensive overview on MB formation mechanism. XRPD/PDF gave information on long/short range behaviors of water desorption/adsorption and indigo sequestration, while TGA and in situ FORS gave information on mass and optical changes within temperature. Ex situ dye removal was used to understand the sample stability after the thermal treatment. A statistical approach based on principal component analysis was exploited to efficiently and jointly analyze the ≈3000 collected patterns. MB formation starts below 110 °C with disordered distribution of indigo within the channels, reaching maximum reaction speed and higher ordering at 150 °C. Above 175 °C, color changes and a stronger sequestration of indigo into framework channels are observed, whereas the affinity for water is dramatically reduced. The origin of different colors, hues, and stability in historical MB samples can then be explained in terms of different thermal histories of the starting mechanical indigo/palygorskite mixtures.}, keywords = {}, pubstate = {published}, tppubtype = {article} } Abstract The exact recipe to prepare the ancient Maya Blue (MB), an incredibly resistant and brilliant pigment prepared from indigo (dye) and Palygorskite (clay), is lost to the ages. To unravel the key features of the MB formation process, several inorganic-dye couples were heated to 200 °C and cooled to RT, to investigate their reactivity and the diffusion and degree of sequestration of the dye into the inorganic host. In situ XRPD/PDF and fiber optic reflectance spectroscopy (FORS) data, along with TGA, provided a comprehensive overview on MB formation mechanism. XRPD/PDF gave information on long/short range behaviors of water desorption/adsorption and indigo sequestration, while TGA and in situ FORS gave information on mass and optical changes within temperature. Ex situ dye removal was used to understand the sample stability after the thermal treatment. A statistical approach based on principal component analysis was exploited to efficiently and jointly analyze the ≈3000 collected patterns. MB formation starts below 110 °C with disordered distribution of indigo within the channels, reaching maximum reaction speed and higher ordering at 150 °C. Above 175 °C, color changes and a stronger sequestration of indigo into framework channels are observed, whereas the affinity for water is dramatically reduced. The origin of different colors, hues, and stability in historical MB samples can then be explained in terms of different thermal histories of the starting mechanical indigo/palygorskite mixtures. | |
Scarfiello, Riccardo; Cesari, Andrea; Altamura, Davide; Masi, Sofia; Nobile, Concetta; Balzano, Federica; Giannini, Cinzia; Grillo, Vincenzo; Tavabi, Amir H; Dunin-Borkowski, Rafal E; Uccello-Barretta, Gloria; Cozzoli, Davide P; Rizzo, Aurora Mechanistic insight into the formation of colloidal WS2 nanoflakes in hot alkylamine media Journal Article Nanoscale Adv., 1 , pp. 2772-2782, 2019. @article{C9NA00279K, title = {Mechanistic insight into the formation of colloidal WS2 nanoflakes in hot alkylamine media}, author = {Riccardo Scarfiello and Andrea Cesari and Davide Altamura and Sofia Masi and Concetta Nobile and Federica Balzano and Cinzia Giannini and Vincenzo Grillo and Amir H Tavabi and Rafal E Dunin-Borkowski and Gloria Uccello-Barretta and Davide P Cozzoli and Aurora Rizzo}, url = {http://dx.doi.org/10.1039/C9NA00279K}, doi = {10.1039/C9NA00279K}, year = {2019}, date = {2019-05-24}, journal = {Nanoscale Adv.}, volume = {1}, pages = {2772-2782}, publisher = {RSC}, abstract = {Developing convenient and reliable synthetic methodologies for solution processable 2D layered ultrathin nanostructures with lateral size control is one of the major challenges for practical applications. In this study, a rational understanding a long-chain amphiphilic surfactant assisted non-hydrolytic synthesis that is able to generate dimension-controllable 2D-WS2 nanocrystal flakes in a single-step protocol is proposed. The evolution of the starting soft organic\textendashinorganic lamellar template into ultrathin few-layer 2D-WS2 nanostructures with lateral size modulation over a range between 3 and 30 nm is monitored. The initial formation of WS2 nanoseeds occurs in a self-assembled sacrificial precursor source, acting as a template, where larger two-dimensional nanostructures can grow without undergoing significant thickness variation. Overall, the chemical nature and steric hindrance of the alkylamines are essential to modulate the reactivity of such WS2 nanoclusters, which correlate with the lateral size of the resulting nanoflakes.}, keywords = {}, pubstate = {published}, tppubtype = {article} } Developing convenient and reliable synthetic methodologies for solution processable 2D layered ultrathin nanostructures with lateral size control is one of the major challenges for practical applications. In this study, a rational understanding a long-chain amphiphilic surfactant assisted non-hydrolytic synthesis that is able to generate dimension-controllable 2D-WS2 nanocrystal flakes in a single-step protocol is proposed. The evolution of the starting soft organic–inorganic lamellar template into ultrathin few-layer 2D-WS2 nanostructures with lateral size modulation over a range between 3 and 30 nm is monitored. The initial formation of WS2 nanoseeds occurs in a self-assembled sacrificial precursor source, acting as a template, where larger two-dimensional nanostructures can grow without undergoing significant thickness variation. Overall, the chemical nature and steric hindrance of the alkylamines are essential to modulate the reactivity of such WS2 nanoclusters, which correlate with the lateral size of the resulting nanoflakes. | |
Belviso, Benny Danilo ; Caliandro, Rosanna ; Salehi, Shabnam Majidi ; Di Profio, Gianluca ; Caliandro, Rocco Protein Crystallization in Ionic-Liquid Hydrogel Composite Membranes Journal Article Crystals, 9 (5), pp. 253, 2019, ISBN: 073-4352. @article{cryst9050253b, title = {Protein Crystallization in Ionic-Liquid Hydrogel Composite Membranes }, author = {Belviso, Benny Danilo and Caliandro, Rosanna and Salehi, Shabnam Majidi and Di Profio, Gianluca and Caliandro, Rocco}, doi = {10.3390/cryst9050253}, isbn = {073-4352}, year = {2019}, date = {2019-05-17}, journal = {Crystals}, volume = {9}, number = {5}, pages = {253}, abstract = {Protein crystallization is a powerful purification tool. It is the first step for crystallographic structural investigations, and can be preparatory for biotechnological applications. However, crystallizing proteins is challenging and methods to control the crystallization process are needed. Ionic-liquid hydrogel composite membranes (IL-HCMs) have been used here as material capable of supporting protein crystallization and hosting grown crystals. We found that IL-HCMs affect the selection mechanism of glucose isomerase (GI) polymorphs and make GI crystals grow completely immersed into the hydrogel layer. X-ray diffraction studies show that IL ions do not bind to the protein, likely because IL molecules are constrained in the polymeric framework. Our GI crystal structures have been compared with many existing GI crystal structures using multivariate analysis tools, allowing a comprehensive overview of factors determining structural similarities, i.e., temperature variations and external stresses exerted during or after crystal growth, such as dehydration or presence of hydrogel of a different nature. GI crystals grown on IL-HCM fit perfectly in this framework, showing typical features induced by external forces. Overall, protein crystallization by IL-HCMs show potential for biotechnological applications, as it could constitute a natural means for containing crystallized enzymes in working conditions.}, keywords = {}, pubstate = {published}, tppubtype = {article} } Protein crystallization is a powerful purification tool. It is the first step for crystallographic structural investigations, and can be preparatory for biotechnological applications. However, crystallizing proteins is challenging and methods to control the crystallization process are needed. Ionic-liquid hydrogel composite membranes (IL-HCMs) have been used here as material capable of supporting protein crystallization and hosting grown crystals. We found that IL-HCMs affect the selection mechanism of glucose isomerase (GI) polymorphs and make GI crystals grow completely immersed into the hydrogel layer. X-ray diffraction studies show that IL ions do not bind to the protein, likely because IL molecules are constrained in the polymeric framework. Our GI crystal structures have been compared with many existing GI crystal structures using multivariate analysis tools, allowing a comprehensive overview of factors determining structural similarities, i.e., temperature variations and external stresses exerted during or after crystal growth, such as dehydration or presence of hydrogel of a different nature. GI crystals grown on IL-HCM fit perfectly in this framework, showing typical features induced by external forces. Overall, protein crystallization by IL-HCMs show potential for biotechnological applications, as it could constitute a natural means for containing crystallized enzymes in working conditions. | |