X-ray radiation damage in crystallography – XRAD
Agreement on scientific cooperation between CNR and HAS (Hungary)
SHORT DESCRIPTION AND USEFUL INFO:
The optimization of the data acquisition protocols in X-ray macromolecular crystallography is of the utmost importance, especially when long wavelength radiation (1.5 Å and above) are used. The aim of the XRAD project is the development and implementation of robust and flexible data collection protocols, which will be implemented at XRD1 and XRD2 beamlines at ELETTRA synchrotron (Trieste). Central to the project is the implementation and use of the software STAC (STrategic Alignment of Crystals) in the XRD1 and XRD2 beamlines control systems. It will be then possible the implementation of more efficient data acquisition protocols, aiming at collecting complete datasets with reduced sets of images and shorter times. Furthermore, a more flexible crystal orientation will be also possible with the aim of maximizing the anomalous contribution, critically important for datasets displaying weak anomalous signal (S- or P-phasing).
The goniometer Huber and the detector Pilatus 2M at the XRD1 beamline
The goniometer mini-k at the XRD2 beamline
Lysozyme diffraction pattern before (A) and after (B) the crystal reorientation with STAC software.