Doriano Lamba

+39 040 226881 (Secretary)
+39 040 375 8514 (Off.) / +39 040 375 7521 (Lab)
+39 040 375 8355 (XRD-1 Beamline)
Fax +39 040 9221126
E-mail : doriano.lambaATts.ic.cnr.it or doriano.lambaATelettra.trieste.it
Address : Istituto di Cristallografia - C.N.R. Unità Organizzativa di Supporto, Sede di Trieste Area Science Park - Basovizza Strada Statale 14 ,Km. 163.5 I-34149 Trieste - Italy

Present position :

- Deputy Head of the IC-CNR Trieste Oustation and Senior Scientist.

Short CV :

1980 PhD in Chemistry cum laude, University of Rome "La Sapienza", Roma, Italy.
1985-1988 Postdoctoral Research Associate in Carbohydrate Chemistry, Astbury Department of
Biophysics, University of Leeds, Leeds, UK.
1993-1995 Postodoctoral Research Associate in Protein Crystallography, Abteilung für
Strukturforschung, Max Planck Institut für Biochemie, Martinsried bei München,
Germany.
1983-1997 Scientist at IC-CNR, Monterotondo (Roma), Italy.
1997-2005 Scientist at IC-CNR Trieste Outstation and XRD-1@ELETTRA Beamline scientist,
Trieste, Italy
2003-2008 Member of the Steering Committee of IC-CNR
2005-2010 Team leader: Crystallography of Biomolecules and Functional Studies - Enabling
Technologies for Drug Discovery, Molecular Design Dept., CNR
2006- Senior Scientist at IC-CNR Trieste Outstation,Trieste, Italy
2008- Member of the Management Committee "Integrated Structural Biology Infrastructure
for Europe"
2009- Italian Delegate of the International Network Protein Engineering Centres
2010- Deputy Head of the IC-CNR Trieste Outstation, Trieste, Italy

Research activity :

- Structural studies of Acetylcholinesterases and their inhibitors: implications for the design of new anti-Alzheimer drugs
- Structural studies of novel oxime as blood-brain barrier penetrating Acetylcholinesterase
reactivators
- Structural studies of neutralizing antibodies (TrkA-receptor and Nerve Growth Factor)
- Structural and functional studies of painless Nerve Growth Factor mutants
- Structural and functional studies of proNeurotrophins
- Structural studies of microbial enzymes involved in polysaccharides degradation and processing
- Targeting mechanisms of Pseudomonas aeruginosa pathogenesis

Selected publications :

Bartolucci C., Haller L.A., Jordis U., Fels G., Lamba D. "Probing Torpedo californica acetylcholinesterase catalytic gorge with two novel bis-functional galanthamine derivatives". Journal of Medicinal Chemisty 53, 745-751 (2010).

Covaceuszach S., Capsoni S., Marinelli S., Pavone F., Ceci M., Ugolini G., Vignone D., Amato G., Paoletti F., Lamba D., Cattaneo, A. "In vitro receptor binding properties of a "painless" NGF mutein, linked to Hereditary Sensory Autonomic Neuropathy type V". Biochemical Biophysical Research Communications 391, 824-829 (2010).

Paoletti F., Covaceuszach S., Konarev P.V., Gonfloni S., Malerba F., Schwarz E., Svergun D.I., Cattaneo A., Lamba D. "Intrinsic structural disorder of mouse proNGF". Proteins, Structure, Function and Bioinformatics 75, 990-1009 (2009).

Covaceuszach S., Cassetta A., Konarev P. V., Gonfloni S., Ibañez C., Rudolph R., Svergun D.I., Lamba D., Cattaneo A. "Dissecting NGF interactions with TrkA and p75 receptors by structural and functional studies of an anti-NGF neutralizing antibody". Journal of Molecular. Biology 381, 881-896 (2008).

Krastanova I., Guarnaccia C., Zahariev S., Degrassi G., Lamba D. "Heterologous expression, purification, crystallization, X-ray analysis and phasing of the acetyl xylan esterase from Bacillus pumilus". Biochimica et Biophysica Acta - Proteins and Proteomics 1748, 222-230 (2005).

Massa C., Guarnaccia C., Lamba D., Anselmi C. "Insight into the structure of an endopolygalacturonase from the phytopathogen Burkholderia cepacia : A biochemical and computational study". Biochimie 92, 1445-1453 (2010).

Pesaresi A., Lamba D. "Insights into the fatty acid chain length specificity of the carboxylesterase PA3859 from Pseudomonas aeruginosa: a combined structural, biochemical and computational study". Biochimie (2010), doi:10.1016/j.biochi.2010.09.001, in press.

Cattaneo A., Covaceuszach S., Lamba D. "Method for the humanization of antibodies and humanized antibodies thereby obtained". Patent WO/2005/061540.