TITLE |
Structural basis for transcription elongation |
STAFF |
Cecilia Bartolucci |
CNR MODULE |
PM.P06.001.001 Cristallography of Biomolecules and Functional Studies |
KEYWORDS |
RNA polymerase, Elongation complex, DNA-damage, Structural Biology |
COLLABORATIONS |
Max-Planck-Institut für Biochemie, München (www.biochem.mpg.de) Ludwig Maximilian Universität, München |
DESCRIPTION |
DNA transcription by DNA-dependent RNA polymerases (RNAP) can be divided into three stages: initiation, elongation and termination. Elongation is highly processive, its different steps between synthesis, halting, substrate loading and RNA release depending on the interactions between RNAP, the nucleic acids signals and auxiliary transcription factors within the elongation complex (EC). [1] Cramer P. Multisuibunit RNA polymerases. Curr. Opin. Struct. Biol. 12, 89-97, 2002.
Elongation complex with an open transcription bubble, modeled on the crystal structure of the Taq core polymerase. Different subunits of the enzyme are shown in different colors. The DNA template strand is gold; the nontemplate strand green and the RNA transcript violett.
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CONTACTS |
Dr. Cecilia Bartolucci Email : cecilia.bartolucciATic.cnr.it Tel. : +39 0690 672 673 |
Last Updated (Friday, 19 November 2010 15:05)