Modelling And Imaging of Bone Tissue Microdiffraction Signals

 

TITLE

Modelling And Imaging of Bone Tissue Microdiffraction Signals

STAFF

Antonietta Guagliardi
Cinzia Giannini
Massimo Ladisa
Giuseppe Chita
Rocco Lassandro

CNR MODULE

Commessa: PM.P04.011 / Diffrazione e imaging a raggi x per l'ingegneria di materiali nanostrutturati e tessuti biologici e per la biodiagnostica
Modulo: PM.P04.011.001 / Sviluppo di metodi e caratterizzazione di materiali cristallini alla nano, micro e mesoscala

KEYWORDS

Bone Tissue Engineering Regenerative Medicine, Biomaterials, Microdiffraction, Debye Function Analysis, Rietveld Analysis, Canonical Correlation Imaging

COLLABORATIONS
University of Genova, Italy
IFN-CNR, Rome, Italy.
IAC-CNR, Bari, Italy.
DESCRIPTION

Bone is a composite tissue whose unique properties of toughness and stiffness mainly rely on the interplay of a mineral (nanocrystals of hydroxyapatite) and a proteic component (microfibrils of collagen I), organizing themselves in different hierarchical levels. Since decades, bone has been studied by diffraction, small angle scattering and scanning microdiffraction techinques, to get deeper insights on the molecular collagen structure and the mechanism of nucleation, growth and subsequent agglomeration of mineral particles. Understanding such details has become of strategic importance for the engineering of biomimetic materials to be used for the reconstruction of large sized bone defects caused by trauma, cancer surgical resections or malformations. In Europe, more than three millions of patients could benefit by such a regenerative approach. Ceramic and polymeric materials are under investigation as potential bone graft substitutes. Our studies have been applied, up to now, to in vivo bioceramic scaffolds seeded with bone marrow stromal cells. As required, these implants are non toxic, resorbable and able to promote the formation of new bone. X-ray Microdiffraction allows to both extract quantitative properties at different length scales and studying their spatial distribution. At the nanometer scale, the Debye Function Analysis (link Debussy) of the scattering signals from newly formed bone gives information about the crystal structure, size and shape of mineral domains; the Rietveld Analysis of the scaffold signals allows the determination of the selective scaffold resorption. At the micro/millimetre scale, the Canonical Correlation Analysis of all microdiffraction signals allows to extend the information from a small set of images into a 2D map (3D in the near future) of the spatial distribution of the structural, microstructural and compositional properties. Future activity will take advantage from the Microdiffraction Laboratory (link), which is presently at the set-up stage.

MODELLING AND IMAGING OF BONE TISSUE MICRODIFFRACTION SIGNALS - Img

Debye Function Analysis of a microdiffraction pattern from bone of new formation

MODELLING AND IMAGING OF BONE TISSUE MICRODIFFRACTION SIGNALS - Img

Scanning Microdiffraction collection of 2D patterns (left) and Canonical Correlation Map (right) built up using five signal models from new bone and scaffold regions.

 

CONTACTS

 

Guagliardi Antonietta
Email : antonietta.guagliardiATic.cnr.it
Tel : +39 031-2386636

 

Last Updated (Thursday, 02 December 2010 16:21)