The laboratory was conceived and realised to permit the maintenance of the collection of Chlamydomonas reinhardtii mutants acquired from resource centers or generated in our laboratories. It hosts more than 50 different strains, including site-directed and random mutants of the Photosystem II D1 protein, NPQ-mutants, cell-wall deficient and high-light resistant mutants. Among the D1-mutants, there are stains selected for their resistance or sensitivity toward different classes of herbicides, and strains selected for their capacity to tolerate oxidative-stress-associated conditions.
The laboratory is equipped to grow in reproducible manner mixotrophic and heterothrophic algae cultures under controlled conditions of light, temperature and agitation, and to handle the microorganisms under sterile conditions. There are possibilities to perform short- and long-term treatments under different light and temperature regimes.
Temperature and light intensity controlled room;
Laminar flow hood Aura vertical s.d.4 (Bioair Instruments Srl, Siziano-Pavia, Italy);
Photo/thermostatic incubator shaker Certomat BS-1 (Sartorius AG, Goettingen, Germany);
Orbital shaker GFL 3015 (GFL, Burgwedel, Germany);
Automated Cell Counter TC10 (Bio-Rad Laboratories Srl, Segrate, MI, Italy);
Optical microscope (Leitz Diavert, Olympus).
Name and Surname: MAYA DIMOVA LAMBREVA
Unit: IC-CNR, Via Salaria Km 29,300, 00015 Monterotondo (RM)
Tel: +39 0690672921