Institute of Crystallography - CNR

Mass Spectrometry

Two mass spectrometers are operating at the IC spectrometry laboratory in Catania:

MALDI TOF/TOF Mass Spectrometer 5800 AB SCIEX

The AB SCIEX 5800 MALDI is a mass spectrometer with TOF/Reflectron analyzer. It provide improved protein identification results for single spot analysis. The MALDI source enables deeper analysis into each sample, even if mixed with salts, and much faster acquisition. A variable rate 1000Hz laser increases the speed of each acquisition by collecting more spots per unit time. The instrument is equipped with a TOF/reflectron mass analyzer. The Time Of Flight (TOF) analyzer enables an ultra-sensitive analysis. The Reflectron mode improve the high resolution analysis. The extended mass range linear detector provides enhanced sensitivity up to m/z of 300,000. These acquisition features provide high quality MS/MS data with minimized sample consumption (ng).

High Resolution ESI-Mass Spectrometer, Q-Exactive Orbitrap Thermo

The Thermo Fischer Q-Exactive Hybrid Quadrupole-Orbitrap Mass Spectrometer is a mass spectrometer system combining quadrupole precursor ion selection with high-resolution, accurate-mass Orbitrap detection, with electrospray ion source (ESI). The system can be coupled with UHPLC UltiMate 3000 Dionex equipped with an autosampler that allow to analyze a big number of samples in a short time. Capillary and nano columns can be used to small analyte concentration. The flexible capabilities of the Q Exactive instrument allow the identification and characterization of wide spectrum of compounds, ranging from small molecules to large polymers and reaching accuracy (nanomolar), sensitivity and resolution suitable for many applications.

Scientific manager
Technical manager
+39 095 7338 432