- Research and consultancy activities aimed at formulating technical and / or scientific opinions on the characterization of polymeric biomaterials for regenerative medicine;
- Feasibility studies, technical and scientific assistance for the design of innovative biomedical devices; Dissemination and / or training local activities through the planning and / or organization and execution of workshops, seminars, conferences, conventions.
Referent: Dott.ssa Anna Giovanna Sciancalepore
- Molecular dynamics simulations of biological macromolecules for: i) the study of their dynamic properties; ii) the identification of the conformational / functional effect of specific mutations responsible for genetic pathologies.
- In-silico structure-based (molecular docking) and ligand-based (QSAR) studies for the development of predictive models of bioactivity and / or toxicity for drug design and chemical risk prediction (in compliance with the REACH regulation).
- Development of generative models based on artificial intelligence. Development of deep learning models for the de-novo generation of bioactive molecules on specific targets of interest.
Referent:Dott. Giuseppe Felice Mangiatordi
Referent: Dott.ssa Rosanna Rizzi
Our group has a long experience in the crystallization and structural resolution of protein-ligand complexes. The services we provide aim to give atomic resolution structural information of the ligand molecule interacting with the bio-macromolecular target and include:
• final phase of purification of protein samples;
• Evaluation of the quality of the samples by Small Angle X-ray Scattering, electrophoresis and chromatography;
• screening of the crystallization conditions of the protein-ligand complexes by vapor diffusion methods, supported by gel to improve the tolerance of the protein crystals to the soaking conditions;
• Structural determination of protein-binding complexes from X-ray diffraction data.
Referent: Dott. Benny Danilo Belviso
- Scanning X-ray microscopy (min 70 micron spot) with absorption and scattering contrast in transmission geometry (SAXS / WAXS) for atomic and nanometric scale analysis of composite, polymeric and biomaterial.
- Analysis of nanostructured thin films, in reflection (GISAXS / GIWAXS).
Referent: Dott. Davide Altamura
Analysis of X diffraction data for the characterization of materials available in microcrystalline form (powders) for studies on structure / property relationships: structural solution at the atomic level using the EXPO software; qualitative analysis using the QUALX software
Referent: Dott. Angela Altomare
- Molecules Screening and Interaction Study with Surface Plasmon Resonance (SPR)
- Recombinant Protein Expression
- Crystallization, data collection and resolution of protein structures and complexes
- Fluorescence spectroscopy for the functional and structural characterization of macromolecules
- Circular dichroism for the functional and structural characterization of macromolecules
Referent: Dott.ssa Viviana Scognamiglio, Dott.ssa Amina Antonacci
Synthesis, purification and mass characterization of peptides, peptidomimetics and PNA
Referent: Dott.ssa Maria Moccia, Dott.ssa Concetta Avitabile
- X diffraction from polycrystalline sample powders to identify the constituent crystalline phases.
- X-ray fluorescence for the determination of the elements present in the analyzed point. (In the laboratory and on site)
- Radiography of artifacts with X-rays of maximum energy of 90 KeV. (In the laboratory and in situ)
- IR reflectometry of polychrome surfaces with dedicated professional CCD and IR <1050 nm. (In the laboratory and on site)
- UV imaging of polychrome surfaces with CCD and dedicated professional filters (in the laboratory and on site)
- Microfotografia nel visibile di manufatti con microscopio stereoscopico (focus stacking). (In laboratorio e in situ)
Referent: Dott.ssa Ombretta Tarquini
Design and development of custom software for scientific instruments in NI LabView
Referent: Dott. Antonello Ranieri
Characterization of protein folding of peptide conformation and stability studies of proteins by Circular Dichroism measurements.
Referent: Dott. Andrea Caporale
Protein Stability Studies, Ligand Binding and Buffer Selection by DIfferential Scanning Fluorimetry (DSF)
Referent: Dott.ssa Sonia Covaceuszach
Polydispersity of samples in solution by dynamic light scattering (DLS)
Referent: Dott Alessandro Pesaresi
Studies of intermolecular interactions, protein / ligand affinity and thermodynamic characterization of intermolecular interactions by Isothermal Titration Calorimetry (ITC)
Referent: Dott. Alberto Cassetta
Protein / ligand affinity studies (both high and low molecular weight). Protein / ligand interaction kinetics. Residence Time and Library Screening of bioactive compounds by Grating Coupled Interferometry (GCI)
Referent: Dott.ssa Sonia Covaceuszach
Bulk X-ray scattering measurements, grazing incidence X-ray scattering. Single crystal X-ray crystallography (small molecules) and Single crystal X-ray crystallography (Macromolecules / Proteins) by X-ray diffraction XRD1 (synchrotron light)
Referent: Dott.ssa Luisa Barba
