Institute of Crystallography - CNR

KLVFF oligopeptide-decorated amphiphilic cyclodextrin nanomagnets for selective amyloid beta recognition and fishing

Recognition and capture of amyloid beta (Ab) is a challenging task for the early diagnosis of neurodegenerative
disorders, such as Alzheimer’s disease. Here, we report a novel KLVFF-modified nanomagnet
based on magnetic nanoparticles (MNP) covered with a non-ionic amphiphilic b-cyclodextrin (SC16OH) and decorated with KLVFF oligopeptide for the self-recognition of the homologous amino-acids sequence of AB to collect AB (1-42) peptide from aqueous samples. MNP@SC16OH and MNP@SC16OH/Ada-Pep nanoassemblies were fully characterized by complementary techniques both as solid powders and in aqueous dispersions. Single domain MNP@SC16OH/AdaPep nanomagnets of 20-40 nm were observed by TEM analysis. DLS and -potential measurements revealed that MNP@SC16OH nanoassemblies owned in aqueous dispersion a hydrodynamic radius of about 150 nm, which was unaffected by Ada-Pep decoration, while the negative -potential of MNP@SC16OH (-40 mV) became less negative (-30 mV) in MNP@SC16OH/Ada-Pep, confirming the exposition of positively charged KLVFF on nanomagnets surface. The ability of MNP@SC16OH/Ada-Pep to recruit AB (1-42) in aqueous solution was evaluated by MALDI-TOF and compared with the ineffectiveness of undecorated MNP@SC16OH and VFLKF scrambled peptide-decorated nanoassemblies (MNP@SC16OH/Ada-scPep), pointing out the selectivity of KLVFFdecorated nanohybrid towards AB (1-42). Finally, the property of nanomagnets to extract AB in conditioned medium of cells over-producing AB peptides was investigated as proof of concept of effectiveness of these nanomaterials as potential diagnostic tools.

Year
2022
Journal
Journal of colloid and interface science (Print)
Impact factor
9.965
RESEARCH AREA
KEYWORDS
Authors
Mazzaglia, Antonino; Di Natale, Giuseppe; Tosto, Rita; Scala, Angela; Sortino, Giuseppe; Piperno, Anna; Casaletto, Maria Pia; Riminucci, Alberto; Giuffrida, Maria Laura; Mineo, Placido G.; Villari, Valentina; Micali, Norberto; Pappalardo, Giuseppe