Biocatalytic units based on enzyme Crystals for efficient Continuous CO2 Conversion to formate – Bio4C

Global warming and climate change are directly related to Increased atmospheric CO2 levels, which are currently higher than ever
and continue to increase. Current strategies for CO2 reduction are mainly emission mitigation strategies and a new emergency has
forcefully appeared on the international scene: the energy supply crisis triggered by the conflict in Ukraine. In this context, utilizing
CO2 as a carbon source for production of high-in-demand chemicals represents a win-win strategy by both replenishing fossil-fuel
processes and simultaneously assisting in mitigating CO2 accumulation and climate change.
In Bio4C we propose a revolutionary biomimetic system for effective and sustainable CO2 fixation into formate, an energy vector for
biofuel or value-added chemicals generation. The overall goal is to develop a new bio-enabled material capable of effective
conversion of CO2 to alternative fuels and chemicals, where the complex chemical processes carried out in bacteria or living cells
are reproduced by a biomimetic artificial system composed of individual enzymes in their active form, closely packed to reach the
highest possible density and embedded into bacterial-like coats able to preserve the enzymes in working conditions and allowing the
entry of reagents (CO2, HCO3-), the exit of the product (HCOO-) and the exchange of electron-transfer mediators. Formate
dehydrogenase (FDH) enzymes in the crystal form will be used as catalyst to convert CO2 into formate.

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