Istituto di Cristallografia - CNR

A Deeper Insight in Metal Binding to the hCtr1 N-terminus Fragment: Affinity, Speciation and Binding Mode of Binuclear Cu2+ and Mononuclear Ag+ Complex Species.

Ctr1 regulates copper uptake and its intracellular distribution. The first 14 amino acid
sequence of the Ctr1 ectodomain Ctr1(1-14) encompasses the characteristic Amino Terminal Cu2+
and Ni2+ binding motif (ATCUN) as well as the bis-His binding motif (His5 and His6). We report a
combined thermodynamic and spectroscopic (UV-vis, CD, EPR) study dealing with the formation of
Cu2+ homobinuclear complexes with Ctr1(1-14), the percentage of which is not negligible even in the
presence of a small Cu2+ excess and clearly prevails at a M/L ratio of 1.9. Ascorbate fails to reduce
Cu2+ when bound to the ATCUN motif, while it reduces Cu2+ when bound to the His5-His6 motif
involved in the formation of binuclear species. The histidine diade characterizes the second binding
site and is thought to be responsible for ascorbate oxidation. Binding constants and speciation of Ag+
complexes with Ctr1(1-14), which are assumed to mimic Cu+
interaction with N-terminus of Ctr1(1-14),
were also determined. A preliminary immunoblot assay evidences that the anti-Ctr1 extracellular
antibody recognizes Ctr1(1-14) in a different way from the longer Ctr1(1-25) that encompasses a second
His and Met rich domain.

International journal of molecular sciences (Online)
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Antonio Magrì, Giovanni Tabbì, Irina Naletova, Francesco Attanasio*, Giuseppe Arena and Enrico Rizzarelli