Mangini Vincenzo

Dr. Vincenzo Mangini, currently research fellow at Institute of Crystallography, National Research Council in Bari (Italy), graduated in Pharmaceutical, Veterinary and Medical Biotechnologies at the University of Bari (Italy) in 2009 and received his Ph.D. in Chemical and Molecular Sciences at the same University in 2014. He has multidisciplinary experience in bioinorganic and protein chemistry, structural biology, and crystallography.

During the CNR research fellowship he has mainly worked on the structural characterization of proteins involved in metal trafficking pathways and on the study of protein-ligand interactions and he got expertise in expression and purification of recombinant proteins, protein crystallization, and structure solutions from X-ray diffraction. Previously he worked for four years at Center for Biomolecular Nanotechnologies, Italian Institute of Technology (IIT) on synthesis and characterization of metal nanoparticles of controlled shape and size and on design and preparation of nanoparticles functionalized with proteins and peptides for biomedical applications. Before that he worked as postdoctoral researcher at University of Bari focusing on chemical-physical characterization of protein-protein and protein-nanoparticle interaction in physiopathological mechanisms through spectroscopic and spectrometric approaches, microscopic techniques, and molecular biology assays.
He also has supervised several master students from various fields of study (chemistry, biotechnology, chemical and pharmaceutical technologies).He was awarded the national “Anna Laura Segre 2016” prize from the family of Anna Laura Segre and the Italian Discussion Group on Magnetic Resonance for the study on NMR-based approaches for the evaluation of nanotoxicity in 2016 and the Davide Viterbo and Elio Cannillo Grant from Crystallography Italian Association in 2022.

Since several years, he is involved in many collaboration and works on research projects at the interface between chemistry and biology in the field of structural biology.

His experience includes:
– structural characterization of proteins involved in metal trafficking pathways and on the study of protein-ligand interactions through nuclear magnetic resonance, X-ray crystallography, SAXS and Cryo-EM;
– chemical-physical characterization of protein-ligand and protein-nanoparticle interactions in physio-pathological mechanisms through spectroscopic and spectrometric approaches, microscopic techniques, and molecular biology assays;
– synthesis and characterization of metal nanoparticles (NPs) of controlled shape and size; design, preparation and characterization of coordination compounds and of NPs functionalized with a controlled number of pharmaceutical-interesting proteins for clinical and diagnostic applications;
– in vitro assessment of engineered NPs and compounds, including toxicity and biological activity in 2D and 3D cell cultures.

Moreover, he has high specific knowledge and hands-on experience in expression and purification of recombinant proteins; nuclear magnetic resonance, protein crystallization and structural solution from X-ray diffraction and SAXS; circular dichroism; gel-electrophoresis, immunoblot and immunofluorescence; transmission electron and confocal microscopy; bacterial and mammalian cell cultures.