Institute of Crystallography - CNR

Biosensor laboratory for environmental and biomedical diagnostics

Development of optical and electrochemical biosensors for the detection of analytes of agro-environmental and biomedical interest; selection and structural/functional characterization of bioreceptors by fluorescence spectroscopy and circular dichroism; design/synthesis of artificial molecules; immobilization of bioreceptors on nanomodified substrates.

The biosensor laboratory is equipped with electrochemical, optical, and spectrophotometric instruments, potentiostats and devices for microfluidics, tools for the validation of biosensors, spectrofluorimeters for the development of bioreceptors labeled with fluorescent probes. In particular, the structure allows for studies of circular dichroism, fluorescence spectroscopy, and UV / VIS for the biophysical characterization and the study of interaction between biological macromolecules; development of natural or biomimetic bioreceptors; labeling with fluorescent probes and electroactive mediators, functionalization with nanomaterials (Prussian Blue, Carbon Black, gold nanoparticles) and immobilization on ad hoc substrates (array of gold microelectrodes, electrodes printed in plastic or paper, ITO supports); synthesis of biological hybrids for the construction of optoelectronic devices; optimization of electro-optical transduction systems; development of nano and micro-systems of optical and electrochemical biosensors for the detection of environmental contaminants and for the control of food safety and quality; immunosensors and aptasensors for the detection of biomarkers of biomedical interest.

Reference works

– Bartolucci, C*., Scognamiglio, V., Antonacci, A., Fraceto, L. F. (2022). What makes nanotechnologies applied to agriculture green?. Nano Today, 43, 101389 (IF 20.7).
– De Felice, M., De Falco, M., Zappi, D., Antonacci, A., Scognamiglio, V*. (2022). Isothermal amplification-assisted diagnostics for COVID-19. Biosensors and Bioelectronics, 114101 (IF 10.6).
– Antonacci, A., Attaallah, R., Arduini, F., Amine, A., Giardi, M. T., Scognamiglio, V*. (2021). A dual electro-optical biosensor based on Chlamydomonas reinhardtii immobilised on paper-based nanomodified screen-printed electrodes for herbicide monitoring. Journal of Nanobiotechnology, 19(1), 1-13 (IF 10.4).
– Attaallah, R., Antonacci, A., Mazzaracchio, V., Moscone, D., Palleschi, G., Arduini, F., Scognamiglio, V*. (2020). Carbon black nanoparticles to sense algae oxygen evolution for herbicides detection: Atrazine as a case study. Biosensors and Bioelectronics, 159, 112203 (IF 10.6).