Biophysical Characterization

The Biophysical Characterization Laboratory houses a range of equipment for the analysis of the biophysical properties of proteins as well as intermolecular interactions. Techniques available include:
• UV-visible spectrophotometry;
• Dynamic Light Scattering for molecular size and polydispersity characterization;
• Differential Scanning Fluorimetry to track protein thermal stability and to qualitatively study protein-ligand interaction in HT format;
• Isothermal titration calorimetry by a Malvern MicroCal iTC200 to deliver direct, label-free in solution measurement of all binding parameters (binding constants (KD), reaction stoichiometry (n), enthalpy (∆H) and entropy (ΔS)), providing a complete thermodynamic profile of the molecular interaction between binding partners in their native states;
• Grating Coupled Interferometry by GCI Creoptix Wave, an innovative technique developed for the high-sensitivity kinetics and affinity analysis (KD, kon, koff) of label-free molecular interactions with improved sensitivity levels respect to SPR.