Istituto di Cristallografia - CNR

High-Resolution Conformational Analysis of RGDechi-Derived Peptides Based on a Combination of NMR Spectroscopy and MD Simulations

The crucial role of integrin in pathological processes such as tumor progression and metastasis formation has inspired intense efforts to design novel pharmaceutical agents modulating integrin functions in order to provide new tools for potential therapies. In the past decade, we have investigated the biological proprieties of the chimeric peptide RGDechi, containing a cyclic RGD motif linked to an echistatin C-terminal fragment, able to specifically recognize ?v?3 without cross reacting with ?v?5 and ?IIb?3 integrin. Additionally, we have demonstrated using two RGDechi-derived peptides, called RGDechi1-14 and ?RGDechi, that chemical modifications introduced in the C-terminal part of the peptide alter or abolish the binding to the ?v?3 integrin. Here, to shed light on the structural and dynamical determinants involved in the integrin recognition mechanism, we investigate the effects of the chemical modifications by exploring the conformational space sampled by RGDechi1-14 and ?RGDechi using an integrated natural-abundance NMR/MD approach. Our data demonstrate that the flexibility of the RGD-containing cycle is driven by the echistatin C-terminal region of the RGDechi peptide through a coupling mechanism between the N- and C-terminal regions.

International journal of molecular sciences (Print)
Impact factor
Acconcia C.; Paladino A.; Valle M.D.; Farina B.; Del Gatto A.; Gaetano S.D.; Capasso D.; Gentile M.T.; Malgieri G.; Isernia C.; Saviano M.; Fattorusso R.; Zaccaro L.; Russo L.
Autori IC CNR