Istituto di Cristallografia - CNR

Pesaresi Alessandro


Born in Ancona (Italy) in 1973, Alessandro got his Laurea Degree in “Biological Sciences” at the University of Bologna, in 2000, with a thesis on “Ascorbate-independent electron transfer between cytochrome b56 and a 27 kDa ascorbate peroxidase of bean hypocotyls”.
The following year he moved to the International School of Advanced Studies (SISSA/ISAS), where in 2005 he got his PhD in Functional and Structural Genomics, with a thesis on the structural and functional charachterization of the carboxylesterase PA3859 form P. aeruginosa.

After a short post-doc fellowship at the Centro di Eccellenza per la Cristallografia of the University of Trieste, he had a 3 years post-doc at the Albert Einstein College of Medicine (NY, USA), in the laboratory of Prof. Steven Roderick, where he kept working on the structural and biochemical study of bacterial enzymes as potential target for the development of new antibiotic.

In 2009 Alessandro moved back to Trieste where, eventually, he entered a tenure track as a CNR resaercher within the Institute of Crystallography.


Main scientific interest is centered on the structure-function relationship of enzymes.
The recent development of a completely new method for the study of enzyme kinetic based on the fitting of full reaction time courses,  has moved the paradigm of classic enzymology from the steady-state model to a more close-to-reality model which allows to simulate and predict the enzymatic catalysis in much finer details.

The coupling of this enhanced modeling of enzyme kinetic with the conventional crystallographic and biophysical techniques, allows an unprecedented deep understanding of the actual functioning of the enzyme’s machinery.

Skills span from basic molecular biology and protein chemistry, to enzymology, biocrystallography, and other biophysic methods (DLS, CD, SPR).

Mixed and non-competitive enzyme inhibition: underlying mechanisms andmechanistic irrelevance of the formal two-site model

Alessandro Pesaresi

Journal of enzyme inhibition and medicinal chemistry (Online) (2023) [IF=5.700]

Kinetic Modeling of Time-Dependent Enzyme Inhibition by Pre-Steady-State Analysis of Progress Curves: The Case Study of the Anti-Alzheimer’s Drug Galantamine

Doriano Lamba (1,2) and Alessandro Pesaresi (1)

International journal of molecular sciences (Online) (2022) [IF=6.200]

Kinetic and structural studies on the inhibition of acetylcholinesterase and butyrylcholinesterase by a series of multitarget-directed galantamine-peptide derivatives

Pesaresi A.; Lamba D.; Vezenkov L.; Tsekova D.; Lozanov V.

Chemico-biological interactions (Print) (2022) [IF=5.200]