Institute of Crystallography - CNR

Belviso Benny Danilo


Dr. Belviso Benny Danilo got the degree in Chemistry at the University of Bari (Italy) in 2008, defending a thesis in crystallographic methods entitled “Crystallographic methods for structural solution from powder data by anomalous diffusion”. In 2012, he got the PhD in “Applied Enzymatic and Chemical Synthesis” at the University of Bari by carrying out researches regarding crystallization and structural solution of adducts between proteins involved in proteolysis processes and metal ions. His research activity has prosecuted at the Institute of Crystallography (IC) of Consiglio Nazionale delle Ricerche (CNR), with a three-year post-doctoral fellowship from 2012 to 2015. Between 2015 and 2016, he is post-doctoral fellow at the Center for Biomolecular Science (Nottingham University) for a structural biology project related to the ADAMTS13 protein. From 2016 to 2018 he is back to IC-CNR for a post-doctoral fellowship related to a Horizon2020 project regarding the development of new materials for purification of antibody by crystallization methods. In December 2019, he becomes permanent employee as staff researcher at the IC-CNR.

He spent several periods of study and research abroad:

  • Research period founded by AIC at the Kyungpook National University, Daegu, South Korea, August 2019-October 2019, to carry out crystal structure determination of NSD proteins, guest of Prof. Eric di Luccio;
  • visiting scientist at the Kyungpook National University, Daegu, South Korea, November 19-December 7 2017, to carry out crystal structure determination of epigenetic targets, guest of Prof. Eric di Luccio.
  • Research period founded by CNR-Short term mobility program at the Membrane Protein Lab of the DIAMOND Ltd Synchrotron, Didcot, Uk, September 2013, to perform structural investigation about reaction center and molecular antennae, guest of Prof. Isabel De Moraes.

His studies concern the crystallization and structural characterization of proteins involved in epigenetic processes (NSDs), primary hemostasis (ADAMTS13), proteolysis (Thrombin), copper homeostasis (ATOX1, MNK1) in the presence of endogenous/exogenous metals, and of adducts between novel platinum based anticancer drugs and matrix metallo proteins.

He is member of the AIC and of Società Chimica Italiana (SCI) and it is part of the national teaching committee of AIC as well of the board of SCI Puglia.
He reviewed manuscripts submitted to: Crystals, Antibiotics, Catalysis, Symmetry, and Molecules.
He has been referee for the Italian Scientific Evaluation ANVUR 2015-2019.


Since from the degree period, he has worked in the field of protein crystallography and structural biology. His experience includes crystallization of soluble and membrane proteins and of adducts between proteins and heavy metals, as well as in data collection and analysis if X-ray diffraction, absorption, and scattering data from protein samples. Moreover, he is also skilled in development of composite materials including protein crystals and in the developing of new crystallization methods for protein molecules. He has participated to several X-ray diffraction, absorption, and scattering experiments at the main European Synchrotron facilities (DIAMOND, ELETTRA, ESRF, and SLS).

A workflow for the development of template-assisted membrane crystallization downstream processing for monoclonal antibody purification (15/09/2023)
Antibodies are among the best-selling biopharmaceuticals today. Their cost is significantly influenced by the purification process (from 40 to 60%). Considering the clear returns in terms of competitiveness, many pharmaceutical companies have actively engaged in finding new strategies to reduce the impact of the purification process on the final drug cost. Thanks to AMECRYS project (Horizon 2020, No. 712965), a membrane crystallization-based purification process has been developed, which can significantly reduce antibody purification costs, with a highly positive impact on the final user and the national healthcare system’s cost. The method is potentially applicable to all biopharmaceutical products and has been chosen to be featured on Nature Protocols homepage of the week from 11 to 15th September 2023.

AIC grant for young researcher (01/07/2018)
Structural investigations on new promising targets for epigenetic therapy of cancer diseases: NSD family proteins
DypB peroxidase for aflatoxin removal: New insights into the toxin degradation process

Mangini V, Rosini E, Caliandro R, Mangiatordi GF, Delre P, Sciancalepore AG, Pollegioni L, Haidukowski M, Mazzorana M, Sumarah MW, Renaud JB, Flaig R, Mulè G, Belviso BD, Loi M.

Chemosphere (2023) [IF=8.800]

A workflow for the development of template-assisted membrane crystallization downstream processing for monoclonal antibody purification

Rajoub Nazer, Gerard Charline JJ , Pantuso Elvira, Fontananova Enrica, Caliandro Rocco, Belviso Benny Danilo, Curcio Efrem , Nicoletta Fiore P, Pullen James, Chen Wenqian, Heng Jerry YY, Ruane Sean, Liddell John, Alvey Natalie, ter Horst Joop H, Di Profio Gianluca

Nature protocols (2023)

hERG stereoselective modulation by mexiletine-derived ureas: Molecular docking study, synthesis, and biological evaluation

Milani Gualtiero1, Budriesi Roberta2, Tavazzani Elisa3, Cavalluzzi Maria Maddalena1, Mattioli Laura Beatrice2, Miniero Daniela Valeria4, Delre Pietro5,6, Belviso Benny Danilo6, Denegri Marco3, Cuocci Corrado6, Rotondo Natalie Paola1, De Palma Annalisa4, Gualdani Roberta7, Caliandroro Rocco , Mangiatordi6, Giuseppe Felice6, Kumawat Amit8, Camilloni Carlo8, Priori Silvia3,9,10, Lentini Giovanni1

Archiv der Pharmazie (Weinh.) (2023)

Human aromatic amino acid decarboxylase is an asymmetric and flexible enzyme: implication in AADC deficiency

Bisello Giovanni1, Ribeiro Rui P2, Perduca Massimiliano2, Belviso Benny Danilo3, Polverino de’ Laureto Patrizia4, Giorgetti Alejandro2, Caliandro Rocco3, Bertoldi Mariarita1

Protein science (Online) (2023)

Stretching the chains: the destabilizing impact of Cu2+ and Zn2+ ions on K48-linked diubiquitin

Mangini, Vincenzo; Grasso, Giulia; Belviso, Benny Danilo; Sciacca, Michele F. M.; Lanza, Valeria; Caliandro, Rocco; Milardi, Danilo

Dalton transactions (2003. Print) (2023)

Unveiling the Influence of Hydrated Deep Eutectic Solvents on the Dynamics of Water-Soluble Proteins

Parisse, Gianluca; Narzi, Daniele; Belviso, Benny Danilo; Capriati, Vito; Caliandro, Rocco; Trotta, Massimo; Guidoni, Leonardo

The journal of physical chemistry. B (2023)

New features of the RootProf program for model-free analysis of unidimensional profiles

A. Mazzone, M. Lopresti, B. D. Belviso and R. Caliandro

Journal of applied crystallography (Online) (2023)

Structural Characterization of the Full-Length Anti-CD20 Antibody Rituximab

Belviso B.D.; Mangiatordi G.F.; Alberga D.; Mangini V.; Carrozzini B.; Caliandro R.

Frontiers in Molecular Biosciences (2022)

Enantiomeric Separation and Molecular Modelling of Bioactive 4-Aryl-3,4-dihydropyrimidin-2(1H)-one Ester Derivatives on Teicoplanin-Based Chiral Stationary Phase

Bolognino, Isabella; Carrieri, Antonio; Purgatorio, Rosa; Catto, Marco; Caliandro, Rocco; Carrozzini, Benedetta; Belviso, Benny Danilo; Majellaro, Maria; Sotelo, Eddy; Cellamare, Saverio; Altomare, Cosimo Damiano

Separations (2022)

Crystal Structure of the Human Copper Chaperone ATOX1 Bound to Zinc Ion

Vincenzo Mangini, Benny Danilo Belviso, Maria Incoronata Nardella, Giovanni Natile, Fabio Arnesano, Rocco Caliandro

Biomolecules (2022)

Structure-based drug design of potential inhibitors of FBXW8, the substrate recognition component of Cullin-RING ligase 7

Zhang Y.; Cui L.; Chen W.; Belviso B.D.; Yu B.; Shen Y.

Molecular diversity (2022)

The role of cytochrome c in the chromate reduction by Rhodobacter sphaeroides

Varsalona, Maria; Belviso, Benny Danilo; Labarile, Rossella; Italiano, Francesca; Farinola, Gianluca Maria; Trotta, Massimo; Caliandro, Rocco


Deep Eutectic Solvents A new opportunity for protein crystallization

Benny Danilo Belviso, Filippo Maria Perna, Benedetta Carrozzini, Massimo Trotta, Vito Capriati, Rocco Caliandro


Introducing Protein Crystallization in Hydrated Deep Eutectic Solvents

Benny Danilo Belviso, Filippo Maria Perna, Benedetta Carrozzini, Massimo Trotta,* Vito Capriati,* and Rocco Caliandro

ACS sustainable chemistry & engineering (2021)

Structural Insights into the Vapochromic Behavior of Pt- and Pd-Based Compounds

Belviso BD, Marin F, Fuertes S, Sicilia V, Rizzi R, Ciriaco F, Cappuccino C, Dooryhee E, Falcicchio , Maini L, Altomare A, Caliandro R

Inorganic chemistry (2021)

Three-dimensional structure of human cyclooxygenase (hCOX)-1

Morena Miciaccia 1,5 , Benny Danilo Belviso 2,5 , Mariaclara Iaselli 1 , Gino Cingolani 3 , Savina Ferorelli 1 , Marianna Cappellari 4 , Paola Loguercio Polosa 4 , Maria Grazia Perrone 1 , Rocco Caliandro 2* & Antonio Scilimati 1

Scientific reports (Nature Publishing Group) (2021)