Catania

Protein expression

The Protein Expression laboratory contains all the facilities needed to produce heterologous proteins from E. Coli or appropriately engineered bacteria through the common molecular biology techniques. The growth of bacteria is carried out at controlled temperature and under suitable stirring conditions (MaxQ 6000, Thermo Scientific). The isolation of the bacteria from the relative cultures, as…

Cell Biology

The cell biology laboratory is equipped with all instruments necessary for mammalian cell cultures, primary or continuous lines, both of tumor origin and not. The behaviour of single cells as well as cell populations is explored by the use of numerous technical approaches. These include: immunocitological tecniques analyzed by the means of intra/extracellular flow citometry…

Spectroscopy

The spectroscopy laboratory is dedicated to the biophysical characterization of macromolecules of biological interest. The instrumentation in use, associated with that of the thermodynamic laboratory, allows us to provide structural information on the macromolecules in solution and to investigate structure / activity correlations. The techniques used provide information on the secondary structure (CD) and tertiary…

Electron Paramagnetic Resonance laboratory (@UniCT)

Thermodynamic

Two Nano calorimeters and a potentiometric-galvanometric station are operating at the IC spectrometry laboratory in Catania:

Synthesis laboratory

Automated Microwave Peptide Synthesizer Two microwave-assisted peptide synthesizers (Liberty, CEM) are operating at the IC synthesis laboratory in Catania, capable of satisfying the growing demand for synthetic peptides. Microwave-assisted solid-phase peptide synthesis (MW-SPPS) is a very high-speed, high-efficiency technology widely used for research, recently also available on a kilo scale. MW-SPPS is advantageous in terms…

Mass Spectrometry

Two mass spectrometers are operating at the IC spectrometry laboratory in Catania: