Roma

Cultural Heritage

The Cultural Heritage Laboratory is devoted to study and characterize inorganic materials in cultural heritage through chemical-physical analyses, non-invasive (X diffraction from powders: XRPD, X-ray fluorescence: XRF) and imaging (X-ray, IR reflectography, UV imaging and induced fluorescence imaging (i.e., VIL imaging) and optical microscopy with focus stacking. Analyses are performed both in laboratory and in…

Advanced X-ray Innovative Applications Laboratory (Axial)

Advanced X-ray Innovative Applications Laboratory (Axial). Lo strumento è composto da un generatore Spellman DF3, con una potenza massima di 3 Kw erogata attraverso un tubo rX di pari capacità con target al rame. La geometria dual focus è in grado di lavorare su due differenti esperimenti: 1) diffrazione da polveri microcristalline per determinazioni qualitative…

Spectroscopy and microalgae-based biosensors

The laboratory is dedicated to the selection and characterization of new biological recognition elements (e.g. microorganisms, proteins and synthetic macromolecules) for biosensoristic purposes by spectroscopic techniques (fluorescence, absorbance and circular dichroism). For these reasons, the laboratory provide portable equipment (potentiometers and fluorimeters) for the development of optical and electrochemical biosensors for agrifood, environmental, biomedical and…

Algal growth

The laboratory allows for the growth of different algal strains of fresh and marine water sources, as well as their physiological characterization by absorption and fluorescence spectroscopy through main parameters as growth rate, cell count, microscopical observation, analysis of the main photosynthetic pigment content, photosynthetic activity. This activity enables the selection of novel biological recognition…

Photosynthesis

Research is mainly focused on: i) studies of adaptation and acclimation of photochemical reactions toward biotic and abiotic stress factors; ii) unraveling the correlation between structure and functionality of pigment-protein complexes; iii) development of biological assays based on photosynthetic activity (whole cells, extracts and macromolecular assemblies) for the detection of environmental pollutants; studies on the…

Surface Plasmon Resonance

SensiQ Pioneer AE equipment is a fully-automated, high sensitivity, three-channel Surface Plasmon Resonance (SPR) System ideal for small molecule-protein interaction studies, antibody characterization and selection, and the study of protein-protein interaction kinetics.

Biology

The Laboratory of Biology 1 is a multipurpose space fully equipped for: extraction and manipulation of nucleic acids from a wide range of plant, animal, and microbial samples; expression, extraction, purification, and characterization of recombinant proteins; extraction of photosynthetic active macromolecular assemblies; extraction of secondary metabolites with nutraceutical properties; determination of antioxidant capacity in plant…

Molecular analysis and biosensoristic

The laboratory is dedicated to the quantitative analysis of molecular recognition events carried out through the application of PCR and Real-Time PCR techniques for the determination of differential gene expression from different types of samples, and electrochemical techniques for the development of sensors / biosensors. for use in the agro-environmental and medical sectors. Main equipments:…

Biochemistry

BioLogic DuoFlow Systems. These are medium pressure laboratory scale chromatography systems for protein purification. These systems are modular, capable of running at high flow rates (0.05–80 ml/min) with back pressures up to 3,500 psi, with pH monitoring, buffer blending, and column scouting capabilities. It can be used in conjunction with a fraction collector for unattended…

Automatic Synthesis of Oligonucleotides

Expedite Nucleic Acid Synthesis System. Oligonucleotides are synthesized using phosphoramidite chemistry by Expedite 8900 automatic DNA / RNA synthesizer. This synthesizer allows simultaneous synthesis of two different sequences using two columns. The synthesis can be programmed on a scale of 0.05 µmole, 0.2 µmole, 1 µmole and 15 µmole.

Automatic Synthesis of Peptides/PNA and Mass Spectrometry